Pharmaceutical nanocarrier association with chondrocytes and cartilage explants: influence of surface modification and extracellular matrix depletion
| Autor: | Khaled A. Elsaid, Gerard G M D'Souza, Lucas Antônio Miranda Ferreira, Jason T. Machan, A. Liang, T. Truong |
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| Rok vydání: | 2013 |
| Předmět: |
Cartilage
Articular Time Factors Biomedical Engineering Cell-Penetrating Peptides 02 engineering and technology Cell-penetrating peptide Micelle Article Chondrocyte Injections Intra-Articular Extracellular matrix 03 medical and health sciences Chondrocytes Drug Delivery Systems Rheumatology Osteoarthritis medicine Animals Orthopedics and Sports Medicine Cells Cultured Micelles Fluorescent Dyes 030304 developmental biology 0303 health sciences Liposome Dose-Response Relationship Drug Rhodamines Chemistry Cartilage Cell Membrane Anatomy 021001 nanoscience & nanotechnology Bovine Cartilage Extracellular Matrix medicine.anatomical_structure Liposomes Models Animal Biophysics Cattle Nanocarriers 0210 nano-technology |
| Zdroj: | Osteoarthritis and Cartilage. 21(2):377-384 |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2012.11.011 |
| Popis: | SummaryObjectiveTo evaluate cartilage diffusion and isolated chondrocyte association of micelles and liposomes and to determine the effect of cell-penetrating peptide (CPP) surface functionalization and extracellular matrix depletion on chondrocyte association and cartilage diffusion, respectively.MethodsRhodamine-labeled micelles and liposomes were incubated with bovine chondrocytes and cell-associated fluorescence was quantified using flow cytometry. Rhodamine-labeled CPP-modified micelles and liposomes were incubated with chondrocytes and cell-associated fluorescence was compared to unmodified nanocarriers. Rhodamine-labeled micelles and liposomes were incubated with bovine cartilage explants for 1, 2 and 4 h and cartilage-associated fluorescence was compared across groups. Cartilage explants were treated with interleukin-1 alpha (IL-1α) or with 0.25% trypsin. Rhodamine-labeled micelles and liposomes were incubated with control, IL-1 and trypsin-treated explants and cartilage-associated fluorescence was compared across groups.ResultsChondrocyte-associated fluorescence following treatment with micelles was significantly higher (P |
| Databáze: | OpenAIRE |
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