Far-field nanoscopy with reversible chemical reactions
Autor: | Anton Kurz, Dirk-Peter Herten, Michael Schwering, Arnd Sprödefeld, Alexander Kiel, Roland Krämer, Konstantinos Lymperopoulos |
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Rok vydání: | 2010 |
Předmět: |
genetic structures
Chemistry Pulse (signal processing) Analytical chemistry Near and far field Serum Albumin Bovine Astrophysics::Cosmology and Extragalactic Astrophysics General Chemistry DNA Fibroblasts Chemical reaction Molecular physics Fluorescence Catalysis Single Molecule Spectroscopy Mice Microscopy NIH 3T3 Cells Organometallic Compounds Animals Nanotechnology Cattle Copper Fluorescent Dyes |
Zdroj: | Angewandte Chemie (International ed. in English). 50(13) |
ISSN: | 1521-3773 |
Popis: | These methods separate overlapping point-spread functions of nearby objects in time by light-inducedswitching of the fluorescent probe between a bright (“on”)and a dark (“off”) state.This principle is used in a spatially defined manner inSTED microscopy to decrease the size of the point-spreadfunction by a donut-shaped depletion pulse. |
Databáze: | OpenAIRE |
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