Far-field nanoscopy with reversible chemical reactions

Autor: Anton Kurz, Dirk-Peter Herten, Michael Schwering, Arnd Sprödefeld, Alexander Kiel, Roland Krämer, Konstantinos Lymperopoulos
Rok vydání: 2010
Předmět:
Zdroj: Angewandte Chemie (International ed. in English). 50(13)
ISSN: 1521-3773
Popis: These methods separate overlapping point-spread functions of nearby objects in time by light-inducedswitching of the fluorescent probe between a bright (“on”)and a dark (“off”) state.This principle is used in a spatially defined manner inSTED microscopy to decrease the size of the point-spreadfunction by a donut-shaped depletion pulse.
Databáze: OpenAIRE