Insulin-like growth factor-binding protein 7 is up-regulated during EAE and inhibits the differentiation of oligodendrocyte precursor cells
| Autor: | Qi Shao, Li Cao, Dunxin Han, Yingyan Pu, Ming Zhao, Xue Fang, Weixing Tan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Biophysics HES5 Biology Biochemistry Insulin-like growth factor-binding protein Mice Downregulation and upregulation medicine Animals Remyelination Molecular Biology DNA Primers Messenger RNA Base Sequence Multiple sclerosis Experimental autoimmune encephalomyelitis Cell Differentiation Cell Biology medicine.disease In vitro Up-Regulation Cell biology Insulin-Like Growth Factor Binding Proteins Mice Inbred C57BL Oligodendroglia medicine.anatomical_structure Immunology biology.protein Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 460:639-644 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2015.03.082 |
| Popis: | Oligodendrocyte precursor cells (OPCs) differentiation failure is one of the leading causes for remyelination defects in the demyelinating lesions of multiple sclerosis (MS). In this study, we explored the roles of insulin-like growth factor-binding proteins 7 (IGFBP-7) on OPCs differentiation during experimental autoimmune encephalomyelitis (EAE). We first investigated the expression pattern of IGFBP-7 by real-time PCR and immunofluorescence staining. It showed that IGFBP-7 was expressed in astrocytes (ACs), oligodendrocytes (OLs) and neurons both in vitro and in vivo. The mRNA and protein level of IGFBP-7 was also increased in the spinal cord from mice at the peak of EAE disease. Next we found that IGFBP-7 acted as a negatively regulator of the OPCs differentiation. Together, these data suggest that IGFBP-7 was up regulated during EAE and inhibit the transition from OPCs to mature OLs, implying its use as a potential therapeutic target for the treatment of inflammatory demyelinating diseases. |
| Databáze: | OpenAIRE |
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