Neuregulin-1 suppresses muscarinic receptor expression and acetylcholine-activated muscarinic K+ channels in cardiac myocytes
| Autor: | Li Gan, Yun Liu, Gerald D. Fischbach, Kiwita Phillips, Byron D. Ford, Raul Krauss, Mary Anne Mann |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Carbachol Potassium Channels Neuregulin-1 Biophysics Chick Embryo Biochemistry Internal medicine Muscarinic acetylcholine receptor medicine Muscarinic acetylcholine receptor M4 Animals Molecular Biology Cells Cultured Receptor Muscarinic M4 Chemistry Reverse Transcriptase Polymerase Chain Reaction Myocardium Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Cell Biology Muscarinic acetylcholine receptor M1 Receptors Muscarinic Acetylcholine Nicotinic acetylcholine receptor Endocrinology cardiovascular system medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 308(1) |
| ISSN: | 0006-291X |
| Popis: | The neuregulin-1 family of growth factors regulates nicotinic acetylcholine receptor synthesis in skeletal muscle, but its role in cardiac myogenesis remains unclear. Here, we investigate the involvement of neuregulins in the development of cardiac cholinergic responsiveness. Treatment of chick cardiac myocytes with neuregulin-1 inhibited mRNA expression of the M4 muscarinic receptor, but not the M2 receptor. In addition, mRNA levels of GIRK1 were reduced in myocytes by treatment with neuregulin-1. Activation of cholinergic receptors in cultured chick atrial myocytes by carbachol produced an outward potassium current (I(K(ACh))), which was attenuated by 24-48-h pre-treatment with neuregulin-1. These data suggest that neuregulins can regulate cardiac parasympathetic tone and may be involved in the pathogenesis of cardiac arrhythmias and heart failure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect