A novel selectable marker based on Aspergillus niger arginase expression
| Autor: | Kashyap Dave, T. N. Jayashri, Rekha Bisht Sirola, Manmeet Ahuja, Narayan S. Punekar |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Genetic Markers
Genes Fungal Genetic Vectors Gene Expression Bioengineering Heterologous Expression Applied Microbiology and Biotechnology Biochemistry Gene Transformation Induction Gene Knockout Techniques Transformation Genetic Neurospora-Crassa Complementary DNA Saccharomyces-Cerevisiae DNA Fungal Homologous Recombination Selectable marker Expression vector Arginase Base Sequence biology Arginase (Agaa) Disruption Aspergillus Niger Filamentous Fungi Genetic Complementation Test Aspergillus niger Fungal genetics biology.organism_classification Nidulans Repression Transformation (genetics) Nutritional Markers Disruption Fungal Transformation Biotechnology |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | Selectable markers are valuable tools in transforming asexual fungi like Aspergillus niger. An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a novel nutritional marker/selection for transformation. The development of such a marker was successfully achieved in two steps. The single genomic copy of A. niger arginase gene was disrupted by homologous integration of the bar marker. The agaA disruptant was subsequently complemented by transforming it with agaA expression vectors. Both citA and trpC promoters were able to drive the expression of arginase cDNA. Such agaA(+) transformants displayed arginase expression pattern distinct from that of the parent strain. The results are also consistent with a single catabolic route for arginine in this fungus. A simple yet novel arginine-based selection for filamentous fungal transformation is thus described. (C) 2012 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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