Comparative analysis of virulence determinants and mass spectral profiles of Finnish and Lithuanian endodontic Enterococcus faecalis isolates
| Autor: | A. H. Reynaud Af Geijersstam, E. Røslie, V. Peciuliene, Haroun N. Shah, Renata Culak, Markus Haapasalo, Marie Anne Chattaway, Linda Molenaar |
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| Rok vydání: | 2007 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Microbiology (medical) food.ingredient Proteome Virulence Factors Immunology Population Protein Array Analysis Virulence Virginiamycin Microbiology Enterococcus faecalis law.invention food Bacterial Proteins law Humans Gelatinase Agar education General Dentistry Finland Gram-Positive Bacterial Infections Polymerase chain reaction Antigens Bacterial Molecular Epidemiology education.field_of_study Membrane Glycoproteins biology Perforin Membrane Proteins Lithuania biology.organism_classification Streptococcaceae Anti-Bacterial Agents Gelatinases Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cytolysin Dental Pulp Cavity Carrier Proteins Periapical Periodontitis |
| Zdroj: | Oral Microbiology and Immunology. 22:87-94 |
| ISSN: | 1399-302X 0902-0055 |
| DOI: | 10.1111/j.1399-302x.2007.00327.x |
| Popis: | Introduction: Putative virulence factors of Enterococcus faecalis have been proposed by several workers and, by analogy, these have been linked to strains of endodontic origin. However, their distribution within the cell population is unknown. In the present study, isolates were taken from the dental root canals of two defined human populations, Lithuanian and Finnish, and examined for a range of virulence properties. In addition, surface-associated molecules and intracellular proteins were compared using matrix-assisted laser desorption-ionization/mass spectrometry (MALDI-TOF-MS) and ProteinChipTM capture/MS (SELDI-TOF-MS), respectively. Methods: Twenty-three Lithuanian and 35 Finnish dental root canal isolates were included. The esp, gelE, ace and efaA genes were detected by polymerase chain reaction, and cytolysin and gelatinase phenotypes were determined by hydrolysis of horse blood agar and gelatine agar, respectively. Protein extracts and surface-associated molecules of whole cells were analysed by SELDI-TOF-MS and MALDI-TOF-MS, respectively. Results: Presence of esp (n = 15), cytolysin (n = 9), ace (n = 55) and efaA (n = 58) was not statistically different in the two samples, whereas gelE and gelatinase production was detected more frequently in the Finnish material (chi-squared, P |
| Databáze: | OpenAIRE |
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