Role of Secondary Attachment Sites in Changing the Specificity of Site-Specific Recombination

Autor:	Andrea György, László Dorgai, Robert A. Weisberg, Edit Rutkai
Jazyk:	angličtina
Rok vydání:	2006
Předmět:	Genetics Recombination Genetic Mutation Integrases Virus Integration Mutant Bacteriophages Transposons and Plasmids Biology Site specificity medicine.disease_cause Microbiology Bacteriophage lambda Attachment Sites Microbiological medicine Site-specific recombination Molecular Biology Recombination
Popis:	We previously proposed that lambdoid phages change their insertion specificity by adapting their integrases to sequences found in secondary attachment sites. To test this model, we quantified recombination between partners that carried sequences from secondary attachment sites catalyzed by wild-type and by mutant integrases with altered specificities. The results are consistent with the model, and indicate differential core site usage in excision and integration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::008739139e051443eaaa5a38ff18f1c7 https://europepmc.org/articles/PMC1447459/ Zobrazit plný text záznamu