| Popis: |
The Tropical Race 4 (TR4) of Fusarium oxysporum f. sp. cubense (FOC) is a threat to theproduction of plantains and bananas in Latin America due to its devastating effect and difficult control.Based on these antecedents, the strain MV 020013 of Trichoderma asperellum Samuels, Lieckfeldt &Nirenberg (Ta.13), from the National Center for Plant and Animal Health (CENSA) in Cuba, was evaluated against a FOC-TR4 isolate at Real Strong AgriCulture Innovention, in the Philippines. Thedual-culture technique was used in a PDA medium at 28°C for 12 days. The inhibition percentage ofpathogen growth on the co-culture was calculated and compared with that of the control without theantagonist. This strain of T. asperellum showed high efficacy in controlling this pathogen. At the sixthday of culture, the percentage of FOC TR4 inhibition was 55.60 %, and, on day 12, the antagoniststrain had overgrown the Fusarium colony and completely inhibited its growth. This makes evidentthe presence of mycoparasitism and the possible release of toxic metabolites to the plant pathogen bythe antagonist. This result shows the potential of the strain Ta. 13 of T. asperellum against the causalagent of Panama Disease TR4, being a promising strain that should be evaluated to determine the formof application, dose and efficacy in the control of this disease under field conditions. |
