T-cell receptor gamma and delta gene rearrangements and junctional region characteristics in south Indian patients with T-cell acute lymphoblastic leukemia
| Autor: | Kamalalayam Raghavan Rajalekshmy, Thangarajan Rajkumar, Nirmala Karunakaran Nancy, Natarajan Sudhakar, Ganapathi Ramanan |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Aging Adolescent CD3 T cell Gene Rearrangement delta-Chain T-Cell Antigen Receptor Molecular Sequence Data India Heteroduplex Analysis Biology Polymerase Chain Reaction law.invention law Acute lymphocytic leukemia medicine Humans Leukemia-Lymphoma Adult T-Cell Nucleotide Child Polymerase chain reaction chemistry.chemical_classification Base Sequence Gene Rearrangement gamma-Chain T-Cell Antigen Receptor T-cell receptor Genes T-Cell Receptor gamma Hematology Gene rearrangement medicine.disease Minimal residual disease Molecular biology Clone Cells medicine.anatomical_structure chemistry Child Preschool Immunology biology.protein Female Gene Deletion Genes T-Cell Receptor delta |
| Zdroj: | American journal of hematology. 82(3) |
| ISSN: | 0361-8609 |
| Popis: | Clonal T-cell receptor (TCR) gamma and delta gene rearrangements were studied in 40 T-ALL cases (pediatrics, 29; adults, 11) using PCR with homo-heteroduplex analysis. At least one clonal TCRG or TCRD rearrangement was detected in 34 (85%) cases. TCR gamma (TCRG) rearrangement was detected in 25 (62.5%) cases that included 16 (55%) pediatrics and 9 (81.8%) adults. TCR delta (TCRD) rearrangement was detected in 14/40 (35%) cases, which included 12 (41%) pediatrics and 2 (18%) adults. The frequency of VgammaI-Jgamma1.3/2.3 was significantly more in adults than pediatrics (81.8% vs. 41.3%, P=0.02). In TCRD, Vdelta1-Jdelta1 was rearranged in 10 (25%) cases. The surface membrane CD3 positive cases are significantly associated with absence of TCRD rearrangements (surface membrane CD3+ TCRdelta- 84% vs. surface membrane CD3- TCRdelta- 48%, P value=0.03). Junctional region sequence analyzed with 10 cases each, of TCRG and TCRD, revealed an average junctional region of 7.4 nucleotides (range 2-18 nucleotides) in TCRG and 27 nucleotides (range 14-42 nucleotides) in TCRD-complete rearrangements. In TCRG, trimming at the ends of Vgamma and Jgamma germline nucleotides resulted in deletion, on an average of 9.2 nucleotides. In TCRD, deletion of nucleotides of the Vdelta and Jdelta gene segments on an average was 3.5 nucleotides. The junctional region of TCRD is more diverse than TCRG; nevertheless, the frequency of TCRG was more than that of TCRD and hence we rely more on TCRG clonal markers to quantitate the minimal residual disease in T-ALL. |
| Databáze: | OpenAIRE |
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