Ovarian tissue features assessed in bovine fetuses after vitrification and xenotransplantation procedures
| Autor: | Robson José de Oliveira Júnior, Ana Beatriz Almeida de Moraes, Giovanna Faria de Moraes, Jéssica Cristina dos Santos Marques, K.A. Alves, Ricarda Maria dos Santos, Bruna de Souza Campos, Elso Donizete Silva Junior, Benner Geraldo Alves, Mayara Mafra Soares, Ruth Magalhães Brito, Blake Hampton Gowen, Ricardo Aurélio Avelar de Freitas, Juliano Bergamo Ronda, Carina Diniz Rocha, Daniel Amaral Gontijo |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Stromal cell endocrine system diseases Xenotransplantation medicine.medical_treatment Transplantation Heterologous Biology Cryopreservation Andrology Follicle Mice Endocrinology Fetus Pregnancy Follicular phase medicine Endocrine system Animals Mice Inbred BALB C Ovary Vitrification Transplantation Animal Science and Zoology Cattle Female Tissue Preservation Developmental Biology |
| Zdroj: | Reproductive biology. 21(4) |
| ISSN: | 2300-732X |
| Popis: | Cryopreservation and transplantation of ovarian tissue are proposed methods for the restoration of endocrine function and reproductive potential. Therefore, this study aimed to evaluate the effects of vitrification and xenotransplantation on follicle viability, activation, stromal cell integrity, vascularization, and micronuclei formation. Bovine fetal ovaries were fragmented and assigned to the following groups: Fresh control (FC), ovarian fragments immediately fixed; Vitrified control (VC), ovarian fragments vitrified; Vitrified xenotransplanted (VX), ovarian fragments vitrified and xenotransplanted; and Fresh xenotransplanted (FX), ovarian fragments xenotransplanted. Ovarian fragments were grafted in female BALB/c mice and recovered after 14 days. Follicular viability was preserved (P 0.05) in VC group. The rate of developing follicles was greater (P 0.05) in the FX group compared to other groups. Follicular density was higher (P 0.05) in the VC group than the FC, VX, and FX groups. A decrease (P 0.05) of stromal cell density was recorded after vitrification (VC vs. FX). Blood vessel density decreased in VC, VX, and FX groups compared with the FC group, and blood vessel density was correlated with follicular viability (positively; P = 0.07) and developing follicles (negatively; P 0.001). Both vitrification and xenotransplantation groups (VC, VX, and FX) had a greater (P 0.05) number of cells with one MN compared to the FC group. In summary, our findings showed that both vitrification and xenotransplantation modified blood vessel, follicular and stromal cell densities, follicular viability and activation, and micronuclei formation in ovarian tissue. |
| Databáze: | OpenAIRE |
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