The medical diagnostic approaches with phylogenetic analysis for rare Brucella spp. diagnosis in Taiwan
| Autor: | Yun-Ju Sung, Yun-Ping Lim, Wen-Yu Ho, Yu Ching Lan, Chao-Chin Chang, Bang-Jau You, Yi-Chih Chang, Ni Tien, Michelle Liang, Chen-Mao Ho, Hsiu-Shen Lin, Mao-Wang Ho |
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| Rok vydání: | 2015 |
| Předmět: |
susceptibility test
Medical diagnostic Phylogenetic analysis Phylogenetic tree business.industry Strain (biology) Brucellosis Anti-microbial General Medicine Computational biology Brucella Biology 16S ribosomal RNA medicine.disease biology.organism_classification General Biochemistry Genetics and Molecular Biology Biotechnology Traditional biochemical methods medicine Original Article Identification (biology) 6s rRNA business Bacteria |
| Zdroj: | BioMedicine |
| ISSN: | 2211-8039 |
| DOI: | 10.7603/s40681-015-0009-6 |
| Popis: | Brucellosis is a bacterial zoonotic disease which can be easy to misdiagnose in clinical microbiology laboratories. In the present study, we have tried to improve the current clinical method for detecting Brucella spp. and its antibiotic characteristics. Our method begins with detecting the clinical isolate through traditional biochemical methods and automatic identification systems. Then, we move on to editing the sequence for BLAST allows us to compare 16s rRNA sequences with sequences from other species, allowing the gene level to be determined. Next, the phylogenetic analysis of multiple genetic loci is able to determine the evolutionary relationships between our bacteria strain and those from other locations. Finally, an anti-microbial susceptibility test hones in on the level of antibacterial activity that the bacteria displays. Employing these four steps in concert is extremely effective in identifying rare bacteria. Thus, when attempting to determine the identity of rare bacteria such as Brucella, utilizing these four steps from our research should be highly effective and ultimately prevent further identification errors and misdiagnoses. The standards we have suggested to identify rare bacteria strains is applicable not only to Brucella, but also to other rarely encountered bacteria. |
| Databáze: | OpenAIRE |
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