Dendritic cells as host cells for the promastigote and amastigote stages of Leishmania amazonensis: the role of opsonins in parasite uptake and dendritic cell maturation
| Autor: | Sofiane Zaki Abdi, Mai Lebastard, Emmanuelle Perret, Eric Prina, Jean-Claude Antoine, Nathalie Winter |
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| Přispěvatelé: | Institut Pasteur [Paris], Génétique mycobactérienne - Mycobacterial genetics, Institut Pasteur, Centre National de la Recherche Scientifique, Appel d’offres ‘Puces à ADN 2000-2002’ |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Mice
0302 clinical medicine Phagosomes parasitophorous vacuole Leishmania 0303 health sciences biology phagocytosis CLASS-II MOLECULES Cell Differentiation Flow Cytometry 3. Good health Cell biology SUSCEPTIBLE MICE [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology dendritic cell chemical and pharmacologic phenomena Host-Parasite Interactions 03 medical and health sciences Immune system Animals EPIDERMAL LANGERHANS CELLS Amastigote BALB/C MICE 030304 developmental biology CD86 MHC class II PARASITOPHOROUS VACUOLES maturation Intracellular parasite Macrophages Histocompatibility Antigens Class II Cell Biology Dendritic cell Dendritic Cells IN-VITRO biology.organism_classification Virology amastigote promastigote Microscopy Fluorescence EXPERIMENTAL CUTANEOUS LEISHMANIASIS biology.protein T-CELLS INFECTED MACROPHAGES Lysosomes NORMAL HUMAN-SERUM CD80 030215 immunology |
| Zdroj: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2004, 117 (2), pp.315-325. ⟨10.1242/jcs.00860⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.00860⟩ |
| Popis: | International audience; In their mammalian hosts, Leishmania are obligate intracellular parasites that mainly reside in macrophages. They are also phagocytosed. by dendritic cells (DCs), which play decisive roles in the induction and shaping of T cell-dependent immune responses. Little is known about the role of DCs in the Leishmania life cycle. Here, we examined the ability of mouse bone marrow-derived DCs to serve as hosts for L. amazonensis. Both infective stages of Leishmania (metacyclic promastigotes and amastigotes) could be phagocytosed by DCs, regardless of whether they had previously been experimentally opsonized with either the complement C3 component or specific antibodies. Parasites could survive and even multiply in these cells for at least 72 hours, within parasitophorous vacuoles displaying phagolysosomal characteristics and MHC class II and H-2M molecules. We then studied the degree of maturation reached by infected DCs according to the parasite stage internalised and the type of opsonin used. The cell surface expression of CD24, CD40, CD54, CD80, CD86, OX40L and MHC class II molecules was barely altered following infection with unopsonized promastigotes or amastigotes from nude mice or with C3-coated promastigotes. Even 69 hours post-phagocytosis, a large proportion of infected DCs remained phenotypically immature. In contrast, internalisation of antibody-opsonized promastigotes or amastigotes induced DCs to mature rapidly, as shown by the over-expression of costimulatory, adhesion and MHC class II molecules. Thus, in the absence of specific antibodies (e.g. shortly after infecting naive mammals), infected DCs may remain immature or semi-mature, meaning that they are unable to elicit an efficient anti-Leishmania T cell response. Absence of DC maturation or delayed/incomplete DC maturation could thus be beneficial for the parasites, allowing their establishment and amplification before the onset of immune responses. |
| Databáze: | OpenAIRE |
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