Comparison of european systemic piscine and amphibian iridoviruses with epizootic haematopoietic necrosis virus and frog virus 3
| Autor: | Michel Brémont, W. Ahne, Sandra Essbauer, Monique Béarzotti |
|---|---|
| Přispěvatelé: | ProdInra, Migration, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Amphibian
[SDV]Life Sciences [q-bio] Ranavirus Biology Virus law.invention 03 medical and health sciences chemistry.chemical_compound Fish Diseases law biology.animal Animals Polymerase chain reaction ComputingMilieux_MISCELLANEOUS 030304 developmental biology Antiserum 0303 health sciences Hybridization probe Rana esculenta 04 agricultural and veterinary sciences General Medicine biology.organism_classification Virology Molecular biology DNA Virus Infections 3. Good health Iridoviridae Ictaluridae [SDV] Life Sciences [q-bio] Restriction enzyme chemistry Perches Ictalurus 040102 fisheries 0401 agriculture forestry and fisheries DNA |
| Zdroj: | Zentralblatt für Veterinärmedizin Reihe B Zentralblatt für Veterinärmedizin Reihe B, Wiley-Blackwell, 1998, 45, pp.373-383 |
| ISSN: | 0931-1793 1439-0450 |
| Popis: | Summary Iridovirus-like agents isolated from systemic infected fish (Silurus glanis, SFIR; Ictalurus melas, CFIR I, CFIR II, CFIR III) and from frogs (Rana esculenta, REIR) in Europe, Epizootic Haematopoietic Necrosis Virus (EHNV) isolated in Australia from redfin perch (Perca fluviatilis), and Frog Virus 3 (FV 3) isolated from frogs (Rana pipiens) in the USA were investigated by electron microscopy, polypeptide composition, immunofluorescence, restriction endonuclease digestion, Southern-blot hybridization and polymerase chain reaction (PCR) amplification. All virus isolates proved to be similar in morphology and in size and reacted with EHNV polyclonal antiserum in the immunofluorescence. Whilst DNA restriction profiles of the European piscine isolates cleaved by BamH I were similar, they differed clearly from those of EHNV, REIR and FV 3. Southern-blot analysis of viral BamH I-digested DNA using an EHNV DNA probe revealed cross-hybridization with the DNA of the investigated iridoviruses. Using a set of primers designed for an open reading frame of the EHNV genome, PCR products of about 250 bp were obtained with the DNA of systemic piscine and amphibian iridoviruses. The data suggest that the systemic piscine and amphibian iridoviruses should be regarded as members of the genus Ranavirus within the family Iridoviridae. |
| Databáze: | OpenAIRE |
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