Immunohistochemical localization of S-100β in the dental pulp of the rat molar
| Autor: | Kayoko Nozawa-Inoue, Satoshi Wakisaka, Takehiko Maeda, Kojiro Kurisu, Yukako Atsumi |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Molar Pathology medicine.medical_specialty Immunoelectron microscopy Schwann cell S100 Calcium Binding Protein beta Subunit Rats Sprague-Dawley stomatognathic system medicine Animals Nerve Growth Factors Axon Microscopy Immunoelectron Molecular Biology Dental Pulp Chemistry General Neuroscience Calcium-Binding Proteins S100 Proteins Anatomy Immunohistochemistry Rats stomatognathic diseases medicine.anatomical_structure Odontoblast Dentinal Tubule Axoplasm Pulp (tooth) Neurology (clinical) Developmental Biology |
| Zdroj: | Brain Research. 818:515-519 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(98)01221-9 |
| Popis: | The present study was undertaken to reveal whether S-100alpha or S-100beta or both are present in the nerve fibers in the rat molar tooth pulp. No immunoreactivity for S-100alpha was observed in the molar pulp. In the root pulp, thick smooth-surfaced structures accompanying the blood vessel showed S-100beta-like immunoreactivity (-LI), and occasionally a very few thin beaded elements exhibited S-100beta-LI. In the coronal pulp, S-100beta-like immunoreactive (-IR) structures arborized repeatedly and extensively; they had a predominantly thick, smooth-surfaced appearance, though parts appeared thin and beaded. Numerous thin varicose S-100beta-IR structures ran through the odontoblast cell layer, and further penetrated into the predentin alongside the dentinal tubules. They could be traced for approximately 10-20 micrometers into the predentin from the pulp-predentin border. Immunoelectron microscopy revealed that the Schwann cells in the root pulp showed S-100beta-LI, and that S-100beta-LI was present in the axoplasm as well as Schwann cells in the coronal pulp. The S-100beta-IR axons were rarely surrounded by S-100beta-IR Schwann cells. In the predentin, S-100beta-IR nerve fibers terminated in a position close to the odontoblast processes. The present findings indicate that S-100beta, not S-100alpha, is present in the axon in the dental pulp and predentin as well as in the Schwann cells. |
| Databáze: | OpenAIRE |
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