Effects of Redox Disturbances on Intestinal Contractile Reactivity in Rats Fed with a Hypercaloric Diet
| Autor: | Anderson Fellyp Avelino Diniz, Elba Dos Santos Ferreira, Maria Thaynan de Lima Carvalho, Lydiane Tavares Toscano, Bagnólia Araújo da Silva, Alexandre Sérgio Silva, Fabiana de Andrade Cavalcante, Iara Leão Luna de Souza, Fernando Ramos Queiroga, Patricia Mirella da Silva |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aging medicine.medical_specialty Carbachol Antioxidant Article Subject medicine.medical_treatment 030209 endocrinology & metabolism Ileum medicine.disease_cause Biochemistry Antioxidants Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Malondialdehyde Internal medicine medicine Animals lcsh:QH573-671 Rats Wistar Gastrointestinal tract lcsh:Cytology Smooth muscle layer Cell Biology General Medicine Thiobarbiturates Diet Rats 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Lipid Peroxidation Energy Intake Oxidation-Reduction Oxidative stress Muscle Contraction Research Article medicine.drug |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2018 (2018) |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Few studies have associated the effects of changes in caloric intake and redox disturbances in the gastrointestinal tract. Therefore, the present study aimed at evaluating the hypercaloric diet consumption influence on the contractile reactivity of intestinal smooth muscle, morphology, and oxidative stress of rat ileum. Wistar rats were randomly divided into groups that received a standard diet and fed with a hypercaloric diet for 8 weeks. Animals were euthanized, and the ileum was isolated to isotonic contraction monitoring. Morphology was evaluated by histological staining and oxidative stress by quantification of malondialdehyde levels and total antioxidant activity. Cumulative concentration-response curves to KCl and carbachol were attenuated in rats fed with a hypercaloric diet compared to those that received a standard diet. In addition, an increase in caloric intake promotes a rise in the thickness of the longitudinal smooth muscle layer of rat ileum and tissue malondialdehyde levels, characterizing lipid peroxidation, as well as a decrease in the antioxidant activity. Thus, it was concluded that the consumption of a hypercaloric diet impairs rat intestinal contractility due to mechanisms involving modifications in the intestinal smooth muscle architecture triggered by redox disturbances. |
| Databáze: | OpenAIRE |
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