Functional Genomic Analysis of the Impact of Camelina (Camelina sativa) Meal on Atlantic Salmon (Salmo salar) Distal Intestine Gene Expression and Physiology

Autor: Tiago S. Hori, Matthew L. Rise, Tyler Brown, Derek M. Anderson, Chang Lin Ye, Xi Xue
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Fish Proteins
Atlantic salmon
Camelina sativa
Salmo salar
Physiology
Nerve Tissue Proteins
Aquatic Science
Applied Microbiology and Biotechnology
03 medical and health sciences
Fish meal
Thioredoxins
Transforming Growth Factor beta
Matrix Metalloproteinase 13
medicine
Animals
Oxidoreductases Acting on Sulfur Group Donors
14. Life underwater
RNA
Messenger
Salmo
Oligonucleotide Array Sequence Analysis
Inflammation
Meal
Lamina propria
Camelina meal
biology
Gene Expression Profiling
Functional genomics
Molecular Sequence Annotation
biology.organism_classification
Animal Feed
Camelina
Diet
Intestines
030104 developmental biology
medicine.anatomical_structure
Gene Ontology
Gene Expression Regulation
Distal intestine
Significance analysis of microarrays
Brassicaceae
Original Article
Biotechnology
Zdroj: Marine Biotechnology (New York, N.y.)
ISSN: 1436-2236
1436-2228
Popis: The inclusion of plant meals in diets of farmed Atlantic salmon can elicit inflammatory responses in the distal intestine (DI). For the present work, fish were fed a standard fish meal (FM) diet or a diet with partial replacement of FM with solvent-extracted camelina meal (CM) (8, 16, or 24 % CM inclusion) during a 16-week feeding trial. A significant decrease in growth performance was seen in fish fed all CM inclusion diets (Hixson et al. in Aquacult Nutr 22:615–630, 2016). A 4x44K oligonucleotide microarray experiment was carried out and significance analysis of microarrays (SAM) and rank products (RP) methods were used to identify differentially expressed genes between the DIs of fish fed the 24 % CM diet and those fed the FM diet. Twelve features representing six known transcripts and two unknowns were identified as CM responsive by both SAM and RP. The six known transcripts (including thioredoxin and ependymin), in addition to tgfb, mmp13, and GILT, were studied using qPCR with RNA templates from all four experimental diet groups. All six microarray-identified genes were confirmed to be CM responsive, as was tgfb and mmp13. Histopathological analyses identified signs of inflammation in the DI of salmon fed CM-containing diets, including lamina propria and sub-epithelial mucosa thickening, infiltration of eosinophilic granule cells, increased goblet cells and decreased enterocyte vacuolization. All of these were significantly altered in 24 % CM compared to all other diets, with the latter two also altered in 16 % CM compared with 8 % CM and control diet groups. Significant correlation was seen between histological parameters as well as between five of the qPCR analyzed genes and histological parameters. These molecular biomarkers of inflammation arising from long-term dietary CM exposure will be useful in the development of CM-containing diets that do not have deleterious effects on salmon growth or physiology. Electronic supplementary material The online version of this article (doi:10.1007/s10126-016-9704-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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