Antihypertensive effect of nuatigenin-3-O-β-chacotriose from Solanum sisymbriifolium Lam. (Solanaceae) (ñuatî pytâ) in experimentally hypertensive (ARH+DOCA) rats under chronic administration
| Autor: | M. A. Campuzano, Y. Montalbetti, M.C. Hellión-Ibarrola, J.H. Dölz-Vargas, Yasunori Momose, María L. Kennedy, D.A. Ibarrola, Esteban Ferro, O. Heinichen, Nelson Alvarenga |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Pharmaceutical Science Blood Pressure Crude drug Solanum Plant Roots Drug Administration Schedule law.invention Mice Oral administration law Internal medicine Drug Discovery medicine Animals Saline Antihypertensive Agents Pharmacology biology Plant Extracts Solanum sisymbriifolium Chemistry Reflux Saponins biology.organism_classification Crossover study Triterpenes Rats Surgery Blood pressure Endocrinology Complementary and alternative medicine Paraguay Hypertension Molecular Medicine Phytotherapy Tropanes |
| Zdroj: | Phytomedicine. 18:634-640 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2011.01.012 |
| Popis: | The aim of the study is to assess the hypotensive properties of the hydro-ethanolic crude root extract (CRE), the n-butanol fraction (F(BtOH)) and nuatigenin-3-O-β-chacotriose, from Solanum sisymbriifolium Lam., in adrenal regeneration hypertension+deoxycorticosterone acetate (ARH+DOCA) rats, following a chronic administration. The roots of S. sisymbriifolium Lam. (Solanaceae) were extracted by reflux with ethanol-water 7:3 and the active extract was fractionated by bioassay-guided liquid-liquid separation. Nuatigenin-3-O-β-chacotriose (B(3-1)) was identified as the main hypotensive compound from the crude drug by spectroscopic methods. Immature Wistar rats of both sexes were submitted to both surgery and deoxycorticosterone acetate treatment to obtain adrenal regeneration hypertensive rats (ARH+DOCA). Different groups of experimentally induced hypertensive rats were randomly allotted and received during 16 weeks a daily oral administration of 1% saline solution (0.1 mL/100g body weigh), 100.0 mg/kg of CRE, 10.0, 30.0 and 50.0 mg/kg of F(BtOH), and 1.0 mg/kg of B(3-1), respectively. In addition, two groups of ARH+DOCA rats were randomly assigned to receive either B(3-1) (1.0 mg/kg/day) or 1% of saline solution (0.1 mL/100g body weight/day) for 7 weeks and then a cross over procedure was performed in order to complete the 16th-week treatment. After 16 weeks of oral administration of crude root extract (CRE), butanolic fraction (F(BtOH)) and nuatigenin-3-O-β-chacotriose (B(3-1)) a significant reduction of blood pressure value was induced in hypertensive animals (ARH+DOCA) in comparison to the control group receiving 1% saline solution, at the end of experiment. Administration of B(3-1) (1.0 mg/kg/day p.o.) to ARH+DOCA rats provoked a significant reduction of blood pressure, observed gradually from 5th week (p |
| Databáze: | OpenAIRE |
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