The aprt heterozygote/hemizygote system for screening mutagenic agents allows detection of large deletions
| Autor: | Abdelmajid Belouchi, Karen Messing, Walter Edward Bradley |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Heterozygote
Hypoxanthine Phosphoribosyltransferase endocrine system Health Toxicology and Mutagenesis Mutant Adenine Phosphoribosyltransferase Mutagenesis (molecular biology technique) Biology Toxicology medicine.disease_cause Cell Line chemistry.chemical_compound Cricetinae Genetics medicine Animals Pentosyltransferases Cloning Molecular Molecular Biology Gene Southern blot Mutation Mutagenicity Tests X-Rays Chinese hamster ovary cell Heterozygote advantage Dose-Response Relationship Radiation Aneuploidy Molecular biology Genes chemistry Hemizygote Ethyl Methanesulfonate Chromosome Deletion DNA |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 199:131-138 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(88)90238-2 |
| Popis: | Frequencies of mutation at the hprt and aprt loci in various CHO cell lines were measured after exposure of the cells to ionizing radiation. In D423 and AA8-16, which are aprt +/− heterozygotes, the ratio of hprt − mutants to aprt − mutants ranged from 0.11 to 0.36. In D422 and AA8-5, which are aprt +/0 cell lines in which only one copy of the gene and its flanking sequences is present these ratios were > 5. In contrast, chemical mutagenesis generated mutations at both loci, in all cell lines, at equal frequencies. Southern blot analysis of DNA from hprt − and aprt − mutants of one of the aprt +/− heterozygous lines showed some apparently unaltered genes, some rearrangements and some complete deletions of hprt among hprt − mutants, but only complete deletions of aprt -linked sequences among aprt − mutants. These results strongly suggest that X-ray-induced mutational events are frequently larger than 40 kb (the length of the hprt gene) and that the difference among the frequencies observed at the two loci in the two types of cell lines were due to the presence of essential sequences close the the respective target genes. The combined use of these cell lines in screening environmental mutagens should allow qualitative as well as quantitative analysis of the mutagenic potential of environmental agents. |
| Databáze: | OpenAIRE |
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