MEMS cytometer for porcine oocyte deformation measurement
| Autor: | Aleksandra Pokrzywnicka, Rafał Walczak, Patrycja Śniadek, Natalia Malyszka, Piotr Pawlak, Wojciech Kubicki, Danylo Lizanets |
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| Rok vydání: | 2019 |
| Předmět: |
Microelectromechanical systems
Materials science Mechanical Engineering Microfluidics 02 engineering and technology Biological potential Deformation (meteorology) 021001 nanoscience & nanotechnology Oocyte Electronic Optical and Magnetic Materials medicine.anatomical_structure Mechanics of Materials medicine Electrical and Electronic Engineering 0210 nano-technology Biological system |
| Zdroj: | National Information Processing Institute |
| ISSN: | 1361-6439 0960-1317 |
| DOI: | 10.1088/1361-6439/ab27f1 |
| Popis: | Mechanical properties of cells are indicators of biological potential, quality and presumptive pathological status. Mammalian oocytes are particularly important cells from the point of view of testing their fertilizing potential and performing in vitro fertilization. However, only occasionally are their mechanical parameters measured. A new method for deformation measurement of in vitro-matured porcine oocytes has been developed and presented in this study. The method was used for multiparametric characterization of gametes using a MEMS-type microcytometer and a new methodology for determining cell deformability. The novel chip design, principle of operation, and fabrication processes are described in the study. Using original analysis of microscopic images with the usage of dedicated software and data analysis algorithms, deformation parameters were determined. Results indicate the relationships between parameters under the influence of cell compression and show that the behaviour of oocytes during measurements depends on the morphological quality of the cumulus-oocyte complexes they originated from. |
| Databáze: | OpenAIRE |
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