Long Non-Coding RNA MALAT1 Promotes Acute Cerebral Infarction Through miRNAs-Mediated hs-CRP Regulation
| Autor: | Ruifeng Meng, Lili Teng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Inflammation Nerve Tissue Proteins Comorbidity Transfection 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Downregulation and upregulation Asian People Risk Factors Internal medicine microRNA medicine Human Umbilical Vein Endothelial Cells Humans RNA Small Interfering 3' Untranslated Regions Aged MALAT1 Binding Sites Cerebral infarction business.industry General Medicine Cerebral Infarction Middle Aged medicine.disease Pathophysiology Up-Regulation body regions Blot MicroRNAs 030104 developmental biology Endocrinology C-Reactive Protein Gene Expression Regulation embryonic structures Acute Disease Female RNA Interference RNA Long Noncoding medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Journal of molecular neuroscience : MN. 69(3) |
| ISSN: | 1559-1166 |
| Popis: | The occurrence of cerebral infarction commonly takes atherosclerosis as the pathophysiological basis, accompanied by chronic inflammation. Hypersensitive C-reactive protein (hs-CRP) is an important inflammatory factor involved in the formation of atherosclerosis. This study aims to investigate the regulation of hs-CRP expression by long-chain non-coding RNA (LncRNA) MALAT1 in acute cerebral infarction patients. Plasma levels of LncRNA MALAT1 and hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p in 256 Chinese Han ACI patients and 256 controls were analyzed. HUVECs were transfected with LncRNA MALAT1, MALAT1 NC, and si-MALAT1, respectively. The expression levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p were analyzed. Then, HUVECs were transfected with hsa-miR-145-5p inhibitor, hsa-miR-140-5p inhibitor, hsa-miR-483-3p inhibitor, hsa-miR-338-3p inhibitor, and hsa-miR-145-5p mimic, hsa-miR-140-5p mimic, hsa-miR-483-3p mimic, hsa-miR-338-3p mimic, and the expression level of hs-CRP was detected by Western blotting. The levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p in the plasma of ACI patients were significantly lower than those in the control group (p |
| Databáze: | OpenAIRE |
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