Placenta-enriched LincRNAs MIR503HG and LINC00629 decrease migration and invasion potential of JEG-3 cell line

Autor: Dalila Luciola Zanette, Bruna Rodrigues Muys, Luiza Ferreira de Araújo, Anemari Ramos Dinarte-Santos, Greice Andreotti de Molfetta, Anelisa Ramão, Julio C. C. Lorenzi, Wilson A. Silva, Rafaela de Barros Lima e Bueno, Daniel Onofre Vidal, Cleidson de Pádua Alves
Rok vydání: 2016
Předmět:
0301 basic medicine
Embryology
Placenta
lcsh:Medicine
medicine.disease_cause
Biochemistry
0302 clinical medicine
Cell Movement
Pregnancy
Gene expression
Medicine and Health Sciences
Protein Isoforms
PLACENTA
Small nucleolar RNAs
lcsh:Science
Conserved Sequence
Regulation of gene expression
Multidisciplinary
DNA methylation
Reproduction
Chromatin
Gene Expression Regulation
Neoplastic
Nucleic acids
030220 oncology & carcinogenesis
Azacitidine
Female
RNA
Long Noncoding
Epigenetics
Anatomy
DNA modification
Sequence Analysis
Chromatin modification
Research Article
Chromosome biology
Cell biology
Biology
Evolution
Molecular
03 medical and health sciences
Extraction techniques
Cell Line
Tumor
microRNA
medicine
Genetics
Humans
Neoplasm Invasiveness
Non-coding RNA
Molecular Biology Techniques
Sequencing Techniques
Gene
Molecular Biology
Cell Nucleus
Biology and life sciences
Gene Expression Profiling
Choriocarcinoma
lcsh:R
Reproductive System
DNA
medicine.disease
Molecular biology
RNA extraction
Gene regulation
Gene expression profiling
Research and analysis methods
MicroRNAs
030104 developmental biology
Long non-coding RNAs
Nucleic Acid Conformation
RNA
lcsh:Q
Carcinogenesis
Sequence Alignment
Developmental Biology
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0151560 (2016)
Popis: LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues and cancers (CT genes) are well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function and regulation mechanism of MIR503HG and LINC00629 lincRNAs. According to our data, MIR503HG expression was almost exclusive to placenta and LINC00629 was highly expressed in placenta and other reproductive tissues. Further analysis, using a cancer cell lines panel, showed that MIR503HG and LINC00629 were expressed in 50% and 100% of the cancer cell lines, respectively. MIR503HG was expressed predominantly in the nucleus of JEG-3 choriocarcinoma cells. We observed a positively correlated expression between MIR503HG and LINC00629, and between the lincRNAs and neighboring miRNAs. Also, both LINC00629 and MIR503GH could be negatively regulated by DNA methylation in an indirect way. Additionally, we identified new transcripts for MIR503HG and LINC00629 that are relatively conserved when compared to other primates. Furthermore, we found that overexpression of MIR503HG2 and the three-exon LINC00629 new isoforms decreased invasion and migration potential of JEG-3 tumor cell line. In conclusion, our results suggest that lincRNAs MIR503HG and LINC00629 impaired migration and invasion capacities in a choriocarcinoma in vitro model, indicating a potential role in human reproduction and tumorigenesis. Moreover, the MIR503HG expression pattern found here could indicate a putative new tumor biomarker.
Databáze: OpenAIRE
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