Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice

Autor: Tae Heung Sim, Hyun Sam Lee, Young Her, Go Eun Yang, Joon Ha Park, Bora Kim, Ji Hyeon Ahn, Tae-Kyeong Lee, Hyun Sook Kim, Cheolwoo Park, Jae-Chul Lee, Moo-Ho Won, Dae Won Kim, Hyejin Sim
Rok vydání: 2020
Předmět:
Antioxidant
Ultraviolet Rays
medicine.medical_treatment
SOD2
Pharmaceutical Science
medicine.disease_cause
Article
collagen fiber
Antioxidants
Mice
03 medical and health sciences
chemistry.chemical_compound
Laminarin
Superoxide Dismutase-1
0302 clinical medicine
Dermis
Superoxides
Drug Discovery
medicine
oxidative stress
Animals
skin and connective tissue diseases
lcsh:QH301-705.5
Glucans
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
Skin
030304 developmental biology
chemistry.chemical_classification
Glutathione Peroxidase
0303 health sciences
integumentary system
biology
Superoxide Dismutase
Superoxide
Glutathione peroxidase
epidermal thickness
Catalase
Molecular biology
medicine.anatomical_structure
lcsh:Biology (General)
Gene Expression Regulation
chemistry
030220 oncology & carcinogenesis
biology.protein
UVB
laminarin
Oxidative stress
Zdroj: Marine Drugs
Volume 18
Issue 7
Marine Drugs, Vol 18, Iss 345, p 345 (2020)
ISSN: 1660-3397
Popis: A number of studies have demonstrated that marine carbohydrates display anti‐oxidant, anti‐melanogenic, and anti‐aging activities in the skin. Laminarin (LA), a low‐molecular‐weight polysaccharide, is found in brown algae. The benefits of LA in ultraviolet B (UVB) induced photodamage of the skin have not been reported. The aim of this study was to investigate the effects of pre‐treated LA on histopathological changes and oxidative damage in mouse dorsal skin on day 5, following repeated UVB exposure. Histopathology, Western blot analysis and immunohistochemical studies showed that epidermal thickness in the UVB group was significantly increased
however, the thickness in the UVB group treated with LA (LA/UVB group) was less compared with that of the UVB group. Collagen fibers in the dermis of the UVB group were significantly decreased and destroyed, whereas, in the LA/UVB group, the density of collagen fibers was significantly increased compared with that of the UVB group. Oxidative stress due to superoxide anion production measured via dihydroethidium fluorescence staining was dramatically increased in the UVB group, whereas in the LA/UVB group, the oxidative stress was significantly decreased. Expressions of SOD1, glutathione peroxidase and catalase were markedly reduced in the UVB group, whereas in the LA/UVB group, they were significantly higher along with SOD2 than in the control group. Taken together, our results indicate that LA pretreatment prevents or attenuates skin damage, by decreasing oxidative stress and increasing antioxidant enzymes in mouse dorsal skin.
Databáze: OpenAIRE
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