Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells
| Autor: | Maija Risteli, Tuula Salo, Pia Nyberg, Emma Pirilä, Meeri Sutinen, Ritva Heljasvaara, Ilkka Alahuhta, Suvi-Tuuli Vilen, Mari Aikio, Otto Väyrynen, Pirjo Åström |
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| Přispěvatelé: | Department of Oral and Maxillofacial Diseases, Clinicum, HUSLAB, Department of Pathology, Medicum |
| Rok vydání: | 2018 |
| Předmět: |
EXPRESSION
0301 basic medicine MIGRATION INVASION 3122 Cancers FIBRONECTIN Matrix metalloproteinase 9 Motility Organotypic model Matrix metalloproteinase Biology MMP9 Peptides Cyclic MATRIX METALLOPROTEINASES MMPS ACTIVATION Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Invasion In vivo Cell Movement Cell Line Tumor Gene silencing Humans Neoplasm Invasiveness PROTECTIVE ROLES Cell Proliferation Tumour microenvironment Arresten RNA TONGUE CANCER CTT2 Cell Biology 313 Dentistry 3. Good health body regions Gene Expression Regulation Neoplastic 030104 developmental biology Matrix Metalloproteinase 9 Cell culture Gelatinases 030220 oncology & carcinogenesis Cancer research Carcinoma Squamous Cell GROWTH Mouth Neoplasms TUMOR MICROENVIRONMENT |
| Zdroj: | Experimental cell research. 376(1) |
| ISSN: | 1090-2422 |
| Popis: | Pro-tumorigenic activities of matrix metalloproteinase (MMP) 9 have been linked to many cancers, but recently the tumour-suppressing role of MMP9 has also been elucidated. The multifaceted evidence on this subject prompted us to examine the role of MMP9 in the behaviour of oral tongue squamous cell carcinoma (OTSCC) cells. We used gelatinase-specific inhibitor, CTT2, and short hairpin (sh) RNA gene silencing to study the effects of MMP9 on proliferation, motility and invasion of an aggressive OTSCC cell line, HSC-3. We found that the migration and invasion of HSC-3 cells were increased by CTT2 and shRNA silencing of MMP9. Proliferation, in turn, was decreased by MMP9 inhibition. Furthermore, arresten-overexpressing HSC-3 cells expressed increased levels of MMP9, but exhibited decreased motility compared with controls. Interestingly, these cells restored their migratory capabilities by CTT2 inhibition of MMP9. Hence, although higher MMP9 expression could give rise to an increased tumour growth in vivo due to increased proliferation, in some circumstances, it may participate in yet unidentified molecular mechanisms that reduce the cell movement in OTSCC. |
| Databáze: | OpenAIRE |
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