The surface structure seen on gonococci after treatment with CMP-NANA is due to sialylation of surface lipopolysaccharide previously described as a ‘capsule’
| Autor: | J. A. Cole, N. J. Parsons, Alan Curry, D.M. Jones, Andrew J. Fox, Harry Smith, Raquel Demarco de Hormaeche |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Lipopolysaccharides
Ruthenium red Lipopolysaccharide Neuraminidase Biology medicine.disease_cause Microbiology Epitope Epitopes chemistry.chemical_compound Neuraminic acid medicine Microscopy Immunoelectron Cell Membrane Antibodies Monoclonal Ruthenium Red N-Acetylneuraminic Acid Neisseria gonorrhoeae Staining Sialic acid carbohydrates (lipids) Infectious Diseases chemistry Cytidine Monophosphate N-Acetylneuraminic Acid Sialic Acids biology.protein |
| Zdroj: | Microbial Pathogenesis. 11:199-210 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/0882-4010(91)90050-k |
| Popis: | Phenotypic serum resistance of gonococci in urethral exudates is due to sialylation of lipopolysaccharide (LPS) by host cytidine 5′-monophospho- N -acetyl neuraminic acid (CMP-NANA). A surface structure was visible on gonococci [strain BS4 (agar)] that had been stained with ruthenium red after incubation with CMP-NANA. This structure was not visible after neuraminidase treatment, which released sialic acid but not LPS. The LPS profiles of strain BS4 (agar) had another in vivo -selected strain Gc40 (variant D1), were similar. A monoclonal antibody (mAb) which recognises epitope C on the LPS of ‘capsulated’ gonococci was shown by immunoblotting to react with several LPS components, including one of about 4.5 kDa which contains the probable site of sialylation by CMP-NANA. The reactions with the mAb were not affected by growing the strains with CMP-NANA nor by neuraminidase treatment of the sialylated LPS. The mAb also gold-labelled the surface of both strains before and after treatment with CMP-NANA. These data indicate that sialylation by CMP-NANA and staining with ruthenium red renders more visible the surface LPS which, sometimes in the past, has been seen as a ‘capsule’. |
| Databáze: | OpenAIRE |
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