Modulation of CD4 expression on lymphoma cells transplanted to mice fed (n - 3) polyunsaturated fatty acids
| Autor: | Daniel Oth, E. Potworowski, M.E. Bégin, G. Ells, G. Mercier, Hélène-Marie Thérien, Pierre Tremblay |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty Lymphoma Ratón Membrane Fluidity medicine.medical_treatment Intraperitoneal injection Biophysics Biology Biochemistry Basal (phylogenetics) Membrane Lipids Mice Fish Oils Internal medicine medicine Animals Phospholipids chemistry.chemical_classification Cell Membrane Cell Biology medicine.disease Fish oil Antigens Differentiation Dietary Fats Transplantation Endocrinology chemistry CD4 Antigens Fatty Acids Unsaturated CD8 Polyunsaturated fatty acid |
| Zdroj: | Biochimica et biophysica acta. 1027(1) |
| ISSN: | 0006-3002 |
| Popis: | Groups of adult AKR mice were fed well defined fats controlled diet regimens. These consisted of either saturated (beef tallow: ‘BT’) or ( n − 3 ) polyunsaturated (fish oil: ‘FO’) fatty acids supplementation to basal mix mouse food. In other groups, the basal mix was given without any fat supplement (‘NF’). Six weeks or more after the initiation of these diet regimens, mice received intraperitoneal injection of histocompatible RDM-4 lymphoma cells. Ascites RDM-4 tumors were harvested approximately two weeks later, and some of their physicochemical properties were studied. It was repeatedly found that: (1) the tumor grew considerably faster in the FO-fed donor than in the BT- or NF-fed donors; (2) cell membrane fluidity, content of C 20 (n − 3) and of C 22 (n − 3) fatty acids were significantly higher in the FO groups than in both BT and NF groups, while the content of C 20 (n − 6) and 22:4(n − 6) fatty acids was concomitantly decreased; (3) expression of the CD4 cell surface marker was always significantly diminished in the FO groups, whereas other markers such as CD8, H2K, Thy-1 and LFA-1 were not affected. Similar results were obtained, whether fats constituted from 1% to 16% by weight of the food intake. Use of a recently selected line of the RDM-4 lymphoma, exhibiting higher CD4 marker expression, resulted in similar observations. On the other hand, CD4 expression on cells from lymphoid organs of healthy adult AKR mice was not detectably modulated by the dietary fats. |
| Databáze: | OpenAIRE |
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