T cells with dual antigen specificity in T cell receptor transgenic mice rejecting allografts
| Autor: | Elizabeth Simpson, Phillip Chandler, P. Julian Dyson, Margaret Millrain, Anna M. Sponaas |
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| Rok vydání: | 1995 |
| Předmět: |
Graft Rejection
Male Receptors Antigen T-Cell alpha-beta T cell CD3 H-Y Antigen Molecular Sequence Data Immunology Mice Transgenic chemical and pharmacologic phenomena Biology Gene Rearrangement T-Lymphocyte Lymphocyte Activation Mice Mice Inbred AKR Antigen T-Lymphocyte Subsets medicine Animals Immunology and Allergy Cytotoxic T cell Amino Acid Sequence Antigens Viral Alleles Mice Inbred BALB C Base Sequence T-cell receptor H-2 Antigens hemic and immune systems Skin Transplantation Flow Cytometry Molecular biology Mice Inbred C57BL medicine.anatomical_structure T-Cell Receptor Gene biology.protein Female Clone (B-cell biology) Spleen CD8 |
| Zdroj: | European Journal of Immunology. 25:2813-2817 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | Allelic exclusion of T cell receptor (TCR) genes is incomplete: a significant percentage (10-30%) of normal human and mouse peripheral T cells express two surface TCR alpha chains, and a small percentage of peripheral human T cells have been reported to express two surface TCR beta chains. A proportion of thymocytes in TCR transgenic mice rearrange endogenous T cell receptor genes, and peripheral T cells with two TCR alpha chains, transgenic and endogenous, have been reported. T cell clones with more than a single TCR heterodimer on their surface might be expected to show specificity for more than one cognate antigen: we report here a T cell clone with dual antigen specificity, isolated from an F5 TCR influenza nucleoprotein (NP 366-374/Db)-specific transgenic female mouse which had rejected an H-2-matched male skin graft. It was selected in vitro by stimulation with male H-2b spleen cells in the absence of the NP366-374 peptide but has specificity for both H-Y/Db and NP366-374. This contrasted with the single NP366-374/Db specificity shown by a control clone isolated from a Rag1-/- F5 mouse. The dual antigen specificity was associated with the rearrangement of endogenous TCR genes and cell surface expression of these as well as the TCR transgene. |
| Databáze: | OpenAIRE |
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