'Indirect' Acute Islet Allograft Destruction in Nonobese Diabetic Mice Is Independent of Donor Major Histocompatibility Complex and Requires Host B Lymphocytes
| Autor: | Tinalyn Kupfer, K. Pham, Joshua N. Beilke, Jonathan D. Buhrman, Ronald G. Gill |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Islets of Langerhans Transplantation
chemical and pharmacologic phenomena Nod Major histocompatibility complex Major Histocompatibility Complex Pathogenesis Mice Mice Inbred NOD Immunity MHC class I Animals Transplantation Homologous Medicine NOD mice Mice Knockout B-Lymphocytes Mice Inbred BALB C Transplantation geography geography.geographical_feature_category biology business.industry Histocompatibility Antigens Class II Islet Mice Inbred C57BL Immunology biology.protein Surgery business |
| Zdroj: | Transplantation Proceedings. 40:462-463 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2008.01.054 |
| Popis: | Islet allografts are destroyed rapidly in spontaneously diabetic nonobese diabetic (NOD) mice. However, whether this process is more similar to conventional allograft immunity, islet-specific autoimmune pathogenesis, or both remains controversial. In particular, we sought to determine whether C57BI/6 donor islet major histocompatibility complex (MHC) class I or class II expression was required for islet allograft destruction in autoimmune prone NOD mice versus non-autoimmune–prone BALB/c mice. Results show that islet allografts deficient in both MHC I and II are uniformly accepted in BALB/c mice. In sharp contrast, such MHC-deficient allografts were destroyed acutely in spontaneously diabetic NOD mice. Such donor MHC-independent rejection implicates “indirect” (host MHC-restricted) immunity as a pathway responsible for islet injury. To determine whether host NOD B lymphocytes could contribute to indirect graft recognition, wild-type and MHC I/II–deficient allografts were grafted into B-lymphocyte–deficient (μMT) NOD mice. Whereas wild-type NOD mice could reject MHC-I/II–deficient islet allografts, such grafts were all accepted in B-lymphocyte–deficient NOD mice. Taken together, these results indicate that NOD mice are capable of vigorous donor MHC-independent islet allograft rejection not found in non-autoimmune–prone recipients. Importantly, B lymphocytes may play a key role as antigen-presenting cells in this exuberant host ’indirect’ response found in NOD mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect