Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection

Autor: Marc Oria, Maria Gomez Lopez, Mohammad Ronaghi, Slaven Erceg, Shom Shanker Bhattacharya, Mireia García Roselló, Juan Córdoba, Ivana Radojevic, Miodrag Stojkovic, Maria Amparo Perez Arago, Victoria Moreno-Manzano, Francisco-Javier Rodríguez-Jiménez
Přispěvatelé: Generalitat Valenciana, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Ministerio de Sanidad y Consumo (España), Fundació La Marató de TV3
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Pathology
medicine.medical_specialty
Embryonic stem cells
Time Factors
Cell Survival
Cellular differentiation
Green Fluorescent Proteins
Biology
Motor Activity
Transfection
Embryonic Stem Cells/Induced Pluripotent Stem Cells
In vivo
Cell Movement
medicine
Animals
Humans
Remyelination
Progenitor cell
Spinal cord injury
Cells
Cultured
Spinal Cord Injuries
Motor Neurons
Oligodendrocytes
Reverse Transcriptase Polymerase Chain Reaction
Stem cell transplantation
Cell Differentiation
Cell Biology
Anatomy
Recovery of Function
medicine.disease
Spinal cord
Evoked Potentials
Motor
Embryonic stem cell
Oligodendrocyte
Nerve Regeneration
Rats
stomatognathic diseases
Disease Models
Animal
Oligodendroglia
medicine.anatomical_structure
nervous system
Gene Expression Regulation
Molecular Medicine
Neural differentiation
Developmental Biology
Zdroj: Stem Cells (Dayton, Ohio)
STEM CELLS
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
Digital.CSIC. Repositorio Institucional del CSIC
ISSN: 1549-4918
1066-5099
Popis: 9 páginas, 4 figuras.-- et al.
Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including spinal cord injury. This study evaluates the therapeutic effects of transplanted hESC-derived oligodendrocyte progenitors (OPC) and/or motoneuron progenitors (MP) on axonal remyelination and functional recovery of adult rats after complete spinal cord transection. OPC and/or MP were grafted into the site of injury in the acute phase. Based on Basso-Beattie-Bresnahan scores recovery of locomotor function was significantly enhanced in rats treated with OPC and/or MP when compared with control animals. When transplanted into the spinal cord immediately after complete transection, OPC and MP survived, migrated, and differentiated into mature oligodendrocytes and neurons showing in vivo electrophysiological activity. Taken together, these results indicate that OPC and MP derived from hESC could be a useful therapeutic strategy to repair injured spinal cord.
This work was supported by funds for research in the field of Regenerative Medicine from the Regional Government Health Department (Generalitat Valenciana), the Instituto Carlos III belonging to the Spanish Ministry of Health and Consumer Affairs, and through the grants from the Spanish Ministry of Science and Innovation (SAF2007-63193) and La Marato (TV3 070,330).
Databáze: OpenAIRE
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