Simian virus 40 carrying an Escherichia coli suppressor gene
| Autor: | George C. Fareed, Dean H. Hamer, Charles A. Thomas, Dana Davoli |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
DNA Replication
DNA Bacterial Transcription Genetic viruses DNA Recombinant Simian virus 40 Biology Recombinant virus medicine.disease_cause Virus Cell Line law.invention chemistry.chemical_compound Suppression Genetic RNA Transfer Structural Biology law Escherichia coli medicine Molecular Biology Gene Base Sequence DNA Restriction Enzymes Virology Molecular biology RNA Bacterial Nonsense suppressor chemistry Transfer RNA Recombinant DNA Nucleic Acid Conformation Helper Viruses DNA |
| Zdroj: | Journal of Molecular Biology. 112:155-182 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/s0022-2836(77)80137-x |
| Popis: | A fragment of Escherichia coli DNA carrying a nonsense suppressor transfer RNA gene was joined in vitro to a fragment of simian virus 40 DNA containing the origin of viral DNA replication and early gene region. This recombinant molecule was replicated and encapsidated into virions in monkey cells coinfected with suitable helper simian virus 40 DNA. Structural studies showed that the E. coli DNA suffered no observable sequence alterations. Monkey cells infected with the recombinant virus transcribe the bacterial DNA, but fail to synthesize detectable amounts of mature suppressor tRNA. |
| Databáze: | OpenAIRE |
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