Diacylglycerol kinase η colocalizes and interacts with apoptosis signal-regulating kinase 3 in response to osmotic shock
| Autor: | Maho Asami, Yuji Suzuki, Daisuke Takahashi, Fumio Sakane |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Osmotic shock MAP kinase kinase kinase Kinase Chemistry QH301-705.5 Diacylglycerol kinase Biophysics Colocalization QD415-436 Subcellular localization Biochemistry Cell biology C-Raf 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Stress granule 030220 oncology & carcinogenesis c-Raf Biology (General) Apoptosis signal-regulating kinase |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 101006-(2021) |
| ISSN: | 2405-5808 |
| Popis: | Diacylglycerol kinase (DGK) η translocates from the cytoplasm to punctate vehicles via osmotic shock. Apoptosis signal-regulating kinase (ASK) 3 (MAP kinase kinase kinase (MAPKKK) 15) is also reported to respond to osmotic shock. Therefore, in the present study, we examined the subcellular localization of DGKη and ASK3 expressed in COS-7 cells under osmotic stress. We found that DGKη was almost completely colocalized with ASK3 in punctate structures in response to osmotic shock. In contrast, DGKδ, which is closely related to DGKη structurally, was not colocalized with ASK3, and DGKη failed to colocalize with another MAPKKK, C-Raf, even under osmotic stress. The structures in which DGKη and ASK3 localized were not stained with stress granule makers. Notably, DGKη strongly interacted with ASK3 in an osmotic shock-dependent manner. These results indicate that DGKη and ASK3 undergo osmotic shock-dependent colocalization and associate with each other in specialized structures. |
| Databáze: | OpenAIRE |
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