Pathogen-Induced Elicitin Production in Transgenic Tobacco Generates a Hypersensitive Response and Nonspecific Disease Resistance
| Autor: | René Delon, Dominique Roby, Nicole Pamboukdjian, Jean-Louis Verrier, Alain Poupet, Harald Keller, Michel Ponchet, Pierre Ricci |
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| Přispěvatelé: | Station de botanique et de pathologie végétale, Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Unité de gestion du département santé des plantes et environnement |
| Rok vydání: | 1999 |
| Předmět: |
Phytophthora
Erysiphe cichoracearum Hypersensitive response 0106 biological sciences Transgene [SDV]Life Sciences [q-bio] Plant Science Biology Plant disease resistance 01 natural sciences Microbiology Fungal Proteins 03 medical and health sciences Tobacco Botany Promoter Regions Genetic ComputingMilieux_MISCELLANEOUS Plant Diseases Plant Proteins 030304 developmental biology 2. Zero hunger 0303 health sciences Phytophthora cryptogea Algal Proteins fungi Esterases food and beverages Elicitin 04 agricultural and veterinary sciences Cell Biology Plants Genetically Modified biology.organism_classification Immunity Innate Elicitor Plants Toxic Thielaviopsis basicola 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Research Article 010606 plant biology & botany |
| Zdroj: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 1999, 11 (2), pp.223-235 |
| ISSN: | 1040-4651 1532-298X |
| DOI: | 10.2307/3870852 |
| Popis: | The rapid and effective activation of disease resistance responses is essential for plant defense against pathogen attack. These responses are initiated when pathogen-derived molecules (elicitors) are recognized by the host. We have developed a strategy for creating novel disease resistance traits whereby transgenic plants respond to infection by a virulent pathogen with the production of an elicitor. To this end, we generated transgenic tobacco plants harboring a fusion between the pathogen-inducible tobacco hsr 203J gene promoter and a Phytophthora cryptogea gene encoding the highly active elicitor cryptogein. Under noninduced conditions, the transgene was silent, and no cryptogein could be detected in the transgenic plants. In contrast, infection by the virulent fungus P. parasitica var nicotianae stimulated cryptogein production that coincided with the fast induction of several defense genes at and around the infection sites. Induced elicitor production resulted in a localized necrosis that resembled a P. cryptogea-induced hypersensitive response and that restricted further growth of the pathogen. The transgenic plants displayed enhanced resistance to fungal pathogens that were unrelated to Phytophthora species, such as Thielaviopsis basicola, Erysiphe cichoracearum, and Botrytis cinerea. Thus, broad-spectrum disease resistance of a plant can be generated without the constitutive synthesis of a transgene product. |
| Databáze: | OpenAIRE |
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