Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38
| Autor: | Niran Roongsawang, Masaaki Morikawa, Siew Ping Lim, Kenji Washio |
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| Rok vydání: | 2010 |
| Předmět: |
Operon
Mutant Guanosine Tetraphosphate Biology Cell morphology Applied Microbiology and Biotechnology Biochemistry Peptides Cyclic Analytical Chemistry chemistry.chemical_compound Lipopeptides Nonribosomal peptide Pseudomonas Hydrolase Molecular Biology chemistry.chemical_classification Organic Chemistry Lipopeptide Epistasis Genetic General Medicine biology.organism_classification chemistry Genes Bacterial Mutagenesis Pseudomonadales Mutation DNA Transposable Elements bacteria Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 74(5) |
| ISSN: | 1347-6947 |
| Popis: | Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology. |
| Databáze: | OpenAIRE |
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