SUPERIORITY OF FLOURESCENT IN SITU HYBRIDIZATION OVER IMMUNOHISTOCHEMISTRY IN DETECTION OF HER2 GENE IN CARCINOMA OF THE URINARY BLADDER ASSOCIATED WITH AND WITHOUT SCHISTOSOMIASIS
| Autor: | Maha Akl, Khaled Elesaily, Mohamed Badawy, Heba Khalil, Olfat Hammam, Maha Mosaad, Ali El Hindawi, Hossam Al Ganzoury, Mohamed Wishahi |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Receptor ErbB-2 Schistosomiasis In situ hybridization Gene duplication medicine Carcinoma Humans skin and connective tissue diseases neoplasms Gene In Situ Hybridization Fluorescence Aged Urinary bladder Oncogene business.industry Middle Aged medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Urinary Bladder Neoplasms Female business |
| Zdroj: | Journal of the Egyptian Society of Parasitology. 44:719-731 |
| ISSN: | 2090-2549 |
| DOI: | 10.21608/jesp.2014.90367 |
| Popis: | HER2 is an oncogene encoding a type 1 tyrosine kinase growth factor receptor and the role of HER2 in the development of numerous types of human cancer is still understood and correlates with clinical outcome, poor prognosis, it is a predictor factor for poor response to chemotherapy. HER2 overexpression is associated with reduced disease free and overall survival. Patients who have HER2 negative expression have a poor prognosis. The aim of the present study is to explore the accuracy of detection of expression of HER2 protein by two different techniques of immunohistochemistry (IHC) and gene amplification by fluorescent in situ hybridization (FISH). The two techniques were applied to sixty two patients that included different cell types of carcinoma of the bladder, benign bilharzial lesions and control. Characteristics of the 62 patients are: 10 chronic cystitis, 19 squamous cell carcinoma (SCC) with schistosomiasis, 33 urothelial carcinoma (UC) schistosomal and non-schistosomal, ten healthy individuals without schistosomiasis served as controls. Gene amplification of HER2 was done using FISH and protein expression of HER2 by IHC. The study was applied on archival data of formalin-fixed paraffin embedded tissues and patient clinical data and follow up for 5 years. Overexpression of HER2 protein was found in 30/52 (57.7%). Fourteen cases had score of 2+, and sixteen cases had score of 3+. Using FISH technique it showed more accurate detection of HER2 gene as those fourteen cases who had score of 2+ had been found to be 5 out of 14 were positive for gene over expression , the other sixteen who had score of 3+ all were positive for gene amplification. HER2 protein and gene was found to be significantly overexpressed in carcinoma of the bladder in both cell types SCC and UC with or without schistosomiasis compared to the benign lesions and control groups (P |
| Databáze: | OpenAIRE |
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