Early Steps of a Thymic Tumor in SV40 Transgenic Mice: Hyperplasia of Medullary Epithelial Cells and Increased Mature Thymocyte Numbers Disturb Thymic Export
| Autor: | Lucile Miquerol, Sophie Ezine, Bernadette Nabarra, Axel Kahn, Florence Vasseur, Geoffroy de Ribains, Cécile Godard, Catherine Martinon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Male
lcsh:Immunologic diseases. Allergy Pathology medicine.medical_specialty Antigens Polyomavirus Transforming T-Lymphocytes Cellular differentiation Transgene Pyruvate Kinase Immunology Mice Transgenic Simian virus 40 Biology Mice Cell Movement medicine Animals Progenitor cell Promoter Regions Genetic Thymic carcinoma Hyperplasia Cell Differentiation Epithelial Cells Thymus Neoplasms Flow Cytometry medicine.disease Cell biology Disease Models Animal Thymocyte Cell Transformation Neoplastic medicine.anatomical_structure Mature Thymocyte Female Bone marrow lcsh:RC581-607 CD8 Research Article Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 9, Iss 4, Pp 223-231 (2002) Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | Bone marrow progenitors migrate to the thymus, where they proliferate and differentiate into immunologically competent T cells. In this report we show that mice transgenic for SV40 T and t antigens under the control of the L-pyruvate kinase promoter develop, in a first step, thymic hyperplasia of both thymocytes and epithelial cells. Morphological studies (histology, immunohistolabeling and electron microscopy) revealed modifications of the thymic microenvironment and gradual expansion of medullary epithelial cells in 1 month-old mice, taking over the cortical region. Then, a thymic carcinoma develops. Two-color labeling of frozen sections identified the transgene in medullary epithelial cells. Flow cytometry analysis demonstrated a marked increase in mature CD4+and CD8+thymocytes in adult mice (39±10×106in transgenic mice and 12±5×106in age-matched controls). Furthermore, thymocyte export was disturbed. |
| Databáze: | OpenAIRE |
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