Impact of various factors on radioactivity distribution in different DBS papers
| Autor: | Xiao Ren, Zuyu Guo, Manfred Zimmer, Gary T Emmons, Patricia Zane, Tobias Paehler |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Paper Chemical Phenomena Clinical Biochemistry Nanotechnology Analytical Chemistry Humans Distribution (pharmacology) Desiccation General Pharmacology Toxicology and Pharmaceutics Blood Coagulation Blood Specimen Collection Temperature Humidity General Medicine Blood collection Spotting Dried blood spot Medical Laboratory Technology Radioactivity Hematocrit Common distribution Environmental science Laboratories Blood Chemical Analysis Biomedical engineering |
| Zdroj: | Bioanalysis. 2:1469-1475 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.10.96 |
| Popis: | Background: Dried blood spot (DBS) sampling could potentially become the preferred blood collection technique in toxicological and clinical studies. Autoradiography was performed to study compound distribution within a dbs under different conditions using five papers, 31ETF, Grade 226, 903®, FTA® and FTA® Elute. Results: The results showed an uneven distribution in all papers with common distribution patterns regardless of compounds: decreased concentrations along the edge, the volcano effect in the middle and the speckle pattern in the center. Treated papers were more readily influenced by environmental factors. Conclusion: Autoradiography enables visualization of a compound’s distribution and can guide bioanalytical assay development by allowing convenient evaluation of factors, such as choice of paper, spotting volume, punch size, punch location, temperature and humidity. |
| Databáze: | OpenAIRE |
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