Caracterización molecular de cepas invasivas de Streptococcus dysgalactiae subsp. equisimilis. Estudio multicéntrico Argentina 2011-2012
| Autor: | Traverso, Fernando, Blanco, Alejandra, Villalon-Panzano, Pilar, Beratz, Noelia, Saez, Juan Antonio, Lopardo, Horacio, National Collaborative Group for the Study of Streptococci and Related Bacteria |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Bacteremia medicine.disease_cause Agar dilution Estreptococos del grupo C Levofloxacin Drug Resistance Multiple Bacterial Streptococcus dysgalactiae subsp. equisimilis Prevalence Child Estreptococos del grupo G Aged 80 and over Virulence Group G streptococci General Medicine Infección invasiva Middle Aged Body Fluids Electrophoresis Gel Pulsed-Field Child Preschool Female Group C streptococci medicine.drug Microbiology (medical) Adult DNA Bacterial Adolescent Tetracycline Streptococcus pyogenes 030106 microbiology Argentina Erythromycin Biology Microbiology 03 medical and health sciences Young Adult stomatognathic system Streptococcal Infections medicine Humans Invasive infection Aged Arthritis Infectious Soft Tissue Infections Genetic Variation Infant Streptococcus biochemical phenomena metabolism and nutrition biology.organism_classification Virology Penicillin 030104 developmental biology Genes Bacterial Streptococcus dysgalactiae |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) |
| Popis: | [EN] Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin [1 mef(A), 3 erm(TR), 1 mef(A)+erm(TR) and 1 erm(TR)+erm(B)] and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline [2 tet(M), 5 tet(M)+tet(O)]. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLSB phenotype. [ES] Streptococcus dysgalactiae subsp. equisimilis (SDSE) posee factores de virulencia similares a Streptococcus pyogenes y, en consecuencia, produce faringitis e infecciones graves indistinguibles de las generadas por este patógeno clásico. Los objetivos del estudio fueron conocer la prevalencia de SDSE en infecciones invasivas en Argentina, estudiar su diversidad genética, determinar la presencia de genes de virulencia, ensayar su sensibilidad a los antibióticos y conocer los genes de resistencia. Se emplearon métodos convencionales de identificación. La sensibilidad se determinó por difusión, Etest y dilución en agar. Participaron 28 centros de 16 ciudades argentinas. Se obtuvieron 23 aislamientos (16 del grupo G y 7 del grupo C) desde el 1-7-2011 hasta el 30-6-2012. Se registraron 2 muertes en adultos (8,7%). La mayoría de los aislamientos fueron obtenidos de sangre (60,9%). Todos eran portadores de los genes speJ y ssa. Los genotipos más frecuentes fueron stG62647, stG653 y stG840. Se detectaron 19 pulsotipos distintos. Todos los aislamientos fueron sensibles a penicilina y levofloxacina, 6 (26,1%) presentaron resistencia o sensibilidad disminuida a eritromicina (1 mef[A], 3 erm[TR], 1 mef[A] + erm[TR] y 1 erm[TR] + erm[B]) y 7 (30,4%) fueron resistentes o tuvieron sensibilidad disminuida a tetraciclina (2 tet[M], 5 tet[M] + tet[O]). La prevalencia anual en la Argentina fue de al menos 23 infecciones invasivas por SDSE y se observó una amplia diversidad genética. Todos los aislamientos presentaron los genes ssa y speJ. Como en otros estudios, la resistencia a macrólidos (26,1%) estuvo asociada, principalmente, al fenotipo MLSB. Sí |
| Databáze: | OpenAIRE |
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