The potential of mucoadhesive polymer in enhancing efficacy of direct immersion vaccination against Flavobacterium columnare infection in tilapia
| Autor: | Katawut Namdee, Naiyaphat Nittayasut, Nopadon Pirarat, Kunat Suktham, Sirikorn Kitiyodom, Teerapong Yata, Suvimol Surassmo, Channarong Rodkhum, Somrudee Kaewmalun |
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| Rok vydání: | 2019 |
| Předmět: |
Gills
0301 basic medicine food.ingredient Polymers Surface Properties Static Electricity Aquaculture Aquatic Science Flavobacterium Columnaris Microbiology Chitosan Fish Diseases 03 medical and health sciences chemistry.chemical_compound food Antigen Flavobacteriaceae Infections Oral administration Adhesives Immersion medicine Animals Environmental Chemistry Potency Mucous Membrane biology Vaccination Tilapia 04 agricultural and veterinary sciences General Medicine biology.organism_classification medicine.disease 030104 developmental biology Vaccines Inactivated chemistry Bacterial Vaccines Flavobacterium columnare 040102 fisheries 0401 agriculture forestry and fisheries |
| Zdroj: | Fish & Shellfish Immunology. 86:635-640 |
| ISSN: | 1050-4648 |
| Popis: | Vaccination is the most effective approach for prevention of infectious diseases in aquaculture. Although immersion vaccination is more applicable compared to in-feed/oral administration and injection, this method suffers from low potency as the efficiency of uptake of antigens through mucosal membranes is limited. In this study, we have successfully developed a mucoadhesive vaccine delivery system to enhance the efficacy of direct immersion vaccination against Flavobacterium columnare, the causative agent of columnaris disease in red tilapia. A formalin-killed negatively charged, bacterial cell suspension was used to prepare a mucoadhesive vaccine by electrostatic coating with positively charged chitosan. Our results demonstrate that the chitosan-complexed vaccine greatly increases its mucoadhesiveness, thus increasing the chances of vaccine uptake by the gill mucosa and improving the protection obtained against columnaris infection. The surface charge of the chitosan-complexed vaccine was altered from anionic to cationic after chitosan modification. Tilapia were vaccinated with the prepared chitosan-complexed vaccine by immersion. The challenge test was then carried out 30 and 60 days post vaccination, which resulted in a high level of mortalities in the non-vaccinated and uncomplexed vaccine groups. A high relative percentage survival (RPS) of vaccinated fish was noted with the mucoadhesive vaccine. Our results indicated that the naked vaccine failed to protect the fish from columnaris infection, which is consistent with the mucoadhesive assays performed during the study showing that the naked vaccine was unable to bind to mucosal surfaces. This system is therefore an effective method for immersion vaccination in order to deliver the antigen preparation to the mucosal surface membrane of the fish. |
| Databáze: | OpenAIRE |
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