Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient
| Autor: | Paul J. Campagnola, Kevin W. Eliceiri, Steven L. Jacques, Gunnsteinn Hall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Forward scatter Quantitative Biology::Tissues and Organs ocis:(290.4210) Multiple scattering Monte Carlo method ocis:(170.6935) Tissue characterization 02 engineering and technology 01 natural sciences ocis:(120.5820) Scattering measurements ocis:(170.3660) Light propagation in tissues 010309 optics 020210 optoelectronics & photonics Optics ocis:(290.5820) Scattering measurements 0103 physical sciences 0202 electrical engineering electronic engineering information engineering Anisotropy Scattering business.industry Attenuation ocis:(290.0290) Scattering Scattering length Atomic and Molecular Physics and Optics Computational physics Optics of Tissue and Turbid Media Deconvolution business Refractive index Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement. |
| Databáze: | OpenAIRE |
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