MicroRNA-200b is downregulated in colon cancer budding cells
| Autor: | Torben Hansen, Flemming Brandt Sørensen, Jan Lindebjerg, Kirsten Nguyen Knudsen, Boye Schnack Nielsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Colorectal cancer lcsh:Medicine Metastasis 0302 clinical medicine Adenocarcinomas Basic Cancer Research Medicine and Health Sciences lcsh:Science In Situ Hybridization Staining Aged 80 and over Multidisciplinary Invasive Tumors Cell Staining Middle Aged Membrane Staining Oncology 030220 oncology & carcinogenesis Colonic Neoplasms Adenocarcinoma Female Research Article Down-Regulation In situ hybridization Biology Research and Analysis Methods Carcinomas 03 medical and health sciences Cytokeratin Tumor budding microRNA medicine Journal Article Humans Cytoplasmic Staining Aged Colorectal Cancer lcsh:R Cancers and Neoplasms Cancer medicine.disease Nuclear Staining MicroRNAs 030104 developmental biology Specimen Preparation and Treatment Cancer research lcsh:Q |
| Zdroj: | Knudsen, K N, Lindebjerg, J, Nielsen, B S, Hansen, T F & Sørensen, F B 2017, ' MicroRNA-200b is downregulated in colon cancer budding cells ', PLOS ONE, vol. 12, no. 5, e0178564 . https://doi.org/10.1371/journal.pone.0178564 PLoS ONE, Vol 12, Iss 5, p e0178564 (2017) PLoS ONE |
| Popis: | BACKGROUND: The microRNA-200 (miR-200) family acts as a major suppressor of epithelial-mesenchymal transition (EMT). Impaired miR-200 expression may lead to EMT initiation and eventually cancer dissemination. The presence of tumor budding cells (TBC) is associated with metastasis and poor prognosis, and molecular similarities to EMT indicate that these cells may reflect ongoing EMT. The aim of this study was to investigate the expression of miR-200b in budding cells of colon cancer and the relationship with the EMT-markers E-cadherin, β-catenin and laminin-5γ2.MATERIAL & METHODS: MiR-200b was investigated by in situ hybridization in 58 cases of stage II (n = 36) and III colon (n = 22) cancers with tumor budding. Expression of E-cadherin, β-catenin and laminin-5γ2 was examined by immunohistochemistry. A multiplex fluorescence assay combining miR-200b with cytokeratin and laminin-5γ2 was employed on a subset of 16 samples.RESULTS: MiR-200b was downregulated in the TBC at the invasive front of 41 out of 58 (71%) cases. The decline was present in both mismatch satellite stable and instable adenocarcinomas. The majority of cases also showed loss of membranous E-cadherin and increased nuclear β-catenin in the TBC, while laminin-5γ2 expression was upregulated at the invasive front and in the tumor buds of approximately half the adenocarcinomas. However, the miR-200b decline was not statistically associated with the expression of any of the EMT-markers. The miR-200b decline was also documented by multiplex fluorescence. Fourteen out of fifteen cases showed a decrease in miR-200b expression in the majority of the TBC, but no obvious relationship between miR-200b and laminin-5γ2 expression was observed. Conclusion: The findings support the assumption of a miR-200b related downregulation in colon cancer budding cells. Whether miR-200b expression may be of clinical significance awaits further studies. |
| Databáze: | OpenAIRE |
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