Structural basis for the methylation of A1408 in 16S rRNA by a panaminoglycoside resistance methyltransferase NpmA from a clinical isolate and analysis of the NpmA interactions with the 30S ribosomal subunit
| Autor: | Nilofer Husain, Lukasz Koscinski, Jayaraman Seetharaman, Janusz M. Bujnicki, Gordana Maravić-Vlahoviček, Fedora Babić, Sonja Obranić, J. Sivaraman |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Kanamycin Resistance S-Adenosylmethionine Methyltransferase Molecular Sequence Data Ribosome Subunits Small Bacterial Biology Ribosome Methylation 03 medical and health sciences Plasmid Structural Biology RNA Ribosomal 16S Genetics Escherichia coli Humans 30S Protein Footprinting 030304 developmental biology 0303 health sciences antibiotic resistance methyltransferase NpmA aminoglycosides sisomicin kanamycin apramycin tobramycin footprinting NpmA-30S subunit model Base Sequence 030306 microbiology Protein footprinting Adenine Escherichia coli Proteins Methyltransferases S-Adenosylhomocysteine Footprinting 3. Good health Biochemistry |
| Zdroj: | Nucleic Acids Research |
| Popis: | Background: NpmA, a methyltransferase that confers high level-resistance to aminoglycoside antibiotics was identified in an Escherichia coli clinical isolate. It belongs to the kanamycin–apramycin methyltransferase (Kam) family and specifically methylates the 16S rRNA at the N1 position of A1408. NpmA is plasmid-encoded and can be transferred between pathogenic bacteria, therefore posing a threat to the successful use of aminoglycosides in clinical practice. Observations: We determined the structures of apo-NpmA and its complexes with cofacor S-adenosylmethionine and reaction by-product S-adenosylhomocysteine at 2.4, 2.7 and 1.68 Angstrom, respectively. We generated 12 NpmA variants with alanine substitutions of evolutionary conserved amino acids and studied their ability to bind the cofactor, to methylate A1408 in the 30S subunit, and to confer resistance to kanamycin in vivo. Residues D30, W107 and W197 were found to be essential. We have also analyzed the interactions between NpmA and the 30S subunit by footprinting experiments and computational docking. Helices 24, 42 and 44 were found to be the main NpmA-binding site. Both experimental and theoretical analyses suggest that NpmA flips out the target nucleotide A1408 to carry out the methylation. Conclusions: The results presented here will assist in the development of specific NpmA inhibitors that could restore the potential of aminoglycoside antibiotics. |
| Databáze: | OpenAIRE |
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