Post-treatment monitoring by ASCL1/LHX8 methylation analysis in women with HIV treated for cervical intraepithelial neoplasia grade 2/3
| Autor: | Frederique J. Vink, Renske D.M. Steenbergen, Wieke W. Kremer, Birgit I. Lissenberg-Witte, Daniëlle A.M. Heideman, Maaike C.G. Bleeker, Marjolein van Zummeren, Erika Breytenbach, Cathy Visser, Azwidowi Lukhwareni, Chris J.L.M. Meijer, Greta Dreyer |
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| Přispěvatelé: | Pathology, AII - Infectious diseases, Obstetrics and gynaecology, APH - Quality of Care, Epidemiology and Data Science, APH - Methodology, CCA - Cancer Treatment and quality of life |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
LIM-Homeodomain Proteins
Papillomavirus Infections Immunology Uterine Cervical Neoplasms HIV Infections DNA Methylation Uterine Cervical Dysplasia Infectious Diseases Basic Helix-Loop-Helix Transcription Factors Humans Immunology and Allergy Female Prospective Studies Papillomaviridae Follow-Up Studies Transcription Factors |
| Zdroj: | AIDS (London, England), 36(7), 953-961. Lippincott Williams and Wilkins Vink, F J, Steenbergen, R D M, Kremer, W W, Lissenberg-Witte, B I, Heideman, D A M, Bleeker, M C G, van Zummeren, M, Breytenbach, E, Visser, C, Lukhwareni, A, Meijer, C J L M & Dreyer, G 2022, ' Post-treatment monitoring by ASCL1/LHX8 methylation analysis in women with HIV treated for cervical intraepithelial neoplasia grade 2/3 ', AIDS (London, England), vol. 36, no. 7, pp. 953-961 . https://doi.org/10.1097/QAD.0000000000003197 |
| ISSN: | 0269-9370 |
| Popis: | Objective:Women with HIV (WWH) have an increased risk to develop recurrent cervical intraepithelial neoplasia grade 2/3 (rCIN2/3) after treatment compared with HIV-negative women. Therefore, appropriate posttreatment monitoring of WWH is important. This study evaluates the performance of ASCL1 and LHX8 methylation analysis as posttreatment monitoring test in WWH treated for CIN2/3, as alternative to cytology or human papillomavirus (HPV) as follow-up test.Design:Prospective observational cohort study.Methods:WWH treated for CIN2/3 by large loop excision of the transformation zone (LLETZ) (n = 61) were invited for follow-up study visits at 1, 2.5 and 4 years after baseline. Baseline and follow-up cervical scrapes were tested for cytology, HPV and DNA methylation of ASCL1 and LHX8 genes. The performance of these strategies for the detection of rCIN2/3 was evaluated in the first follow-up cervical scrape.Results:Thirteen (21.3%) rCIN2/3 lesions were detected within 4 years of follow-up. In women without rCIN2/3 in follow-up, methylation levels of ASCL1 and LHX8 decreased significantly after LLETZ treatment (P = 0.02 and 0.007, respectively). In women with rCIN2/3, methylation levels remained high after LLETZ treatment. The 4-year rCIN2/3 risk was 4.9% (95% CI: 0.6-16.5) for ASCL1/LHX8-negative women, 8.1% (95% CI: 1.7-21.9) for HPV-negative women and 7.7% (95% CI: 2.1-18.5) for cytology-negative women.Conclusion:A negative ASCL1/LHX8 methylation test in follow-up is associated with a low rCIN2/3 risk and could serve as an objective test of cure and well tolerated alternative for HPV and/or cytology screening in the posttreatment monitoring of WWH. |
| Databáze: | OpenAIRE |
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