Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume
| Autor: | Ruediger Hell, Markus Wirtz, Lisa Molvig, Michel Droux, Linda Tabe |
|---|---|
| Přispěvatelé: | Plant Industry, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), Department of Ophthalmology, Casey Eye Institute, Oregon Health and Science University [Portland] (OHSU), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Physiology [SDV]Life Sciences [q-bio] Arabidopsis plant Plant Science Cysteine synthase 01 natural sciences chemistry.chemical_compound Methionine Gene Expression Regulation Plant Serine Serine O-acetyltransferase ComputingMilieux_MISCELLANEOUS 2. Zero hunger chemistry.chemical_classification 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction SerAT Seed Storage Proteins food and beverages Plants Genetically Modified Research Papers Lupinus Lupinus angustifolius Biochemistry Seeds SAT Sunflower seed OAS Metabolic Networks and Pathways sulphur amino acid Genotype Biology nutritive value 03 medical and health sciences Storage protein Cysteine RNA Messenger Crosses Genetic 030304 developmental biology Cysteine Synthase biology.organism_classification seed storage protein Aspartate chemistry biology.protein sulphur O-Acetylserine Serine O-Acetyltransferase Sulfur 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2010, 61 (3), pp.721-733. ⟨10.1093/jxb/erp338⟩ |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erp338 |
| Popis: | There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosynthesis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F(2) seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein. |
| Databáze: | OpenAIRE |
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