Exercise with low glycogen increases PGC-1α gene expression in human skeletal muscle

Autor: Kent Sahlin, Niklas Psilander, Mikael Flockhart, Per Frank
Rok vydání: 2012
Předmět:
Time Factors
Physiology
Biopsy
AMP-Activated Protein Kinases
Mitochondrion
medicine.disease_cause
p38 Mitogen-Activated Protein Kinases
Quadriceps Muscle
Diet
Carbohydrate-Restricted
chemistry.chemical_compound
Orthopedics and Sports Medicine
Phosphorylation
Heat-Shock Proteins
Cross-Over Studies
Glycogen
Mitochondrial Turnover
General Medicine
Glutathione
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Up-Regulation
medicine.anatomical_structure
medicine.symptom
Muscle Contraction
Muscle contraction
medicine.medical_specialty
Pyruvate dehydrogenase kinase
Protein Serine-Threonine Kinases
Biology
Electron Transport Complex IV
Oxygen Consumption
Physiology (medical)
Internal medicine
medicine
Humans
RNA
Messenger
Protein kinase A
Exercise
Sweden
Analysis of Variance
Public Health
Environmental and Occupational Health
Pyruvate Dehydrogenase Acetyl-Transferring Kinase
Skeletal muscle
Bicycling
Mitochondria
Muscle
Oxidative Stress
Endocrinology
Mitochondrial biogenesis
chemistry
Reactive Oxygen Species
Oxidative stress
Acetyl-CoA Carboxylase
Transcription Factors
Zdroj: European Journal of Applied Physiology. 113:951-963
ISSN: 1439-6327
1439-6319
DOI: 10.1007/s00421-012-2504-8
Popis: Recent studies suggest that carbohydrate restriction can improve the training-induced adaptation of muscle oxidative capacity. However, the importance of low muscle glycogen on the molecular signaling of mitochondrial biogenesis remains unclear. Here, we compare the effects of exercise with low (LG) and normal (NG) glycogen on different molecular factors involved in the regulation of mitochondrial biogenesis. Ten highly trained cyclists (VO(2max) 65 ± 1 ml/kg/min, W max 387 ± 8 W) exercised for 60 min at approximately 64 % VO(2max) with either low [166 ± 21 mmol/kg dry weight (dw)] or normal (478 ± 33 mmol/kg dw) muscle glycogen levels achieved by prior exercise/diet intervention. Muscle biopsies were taken before, and 3 h after, exercise. The mRNA of peroxisome proliferator-activated receptor-γ coactivator-1 was enhanced to a greater extent when exercise was performed with low compared with normal glycogen levels (8.1-fold vs. 2.5-fold increase). Cytochrome c oxidase subunit I and pyruvate dehydrogenase kinase isozyme 4 mRNA were increased after LG (1.3- and 114-fold increase, respectively), but not after NG. Phosphorylation of AMP-activated protein kinase, p38 mitogen-activated protein kinases and acetyl-CoA carboxylase was not changed 3 h post-exercise. Mitochondrial reactive oxygen species production and glutathione oxidative status tended to be reduced 3 h post-exercise. We conclude that exercise with low glycogen levels amplifies the expression of the major genetic marker for mitochondrial biogenesis in highly trained cyclists. The results suggest that low glycogen exercise may be beneficial for improving muscle oxidative capacity.
Databáze: OpenAIRE
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