Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode
| Autor: | Cristiane Aparecida Pereira, Milton Beltrame Junior, Antonio Olavo Cardoso Jorge, José Chibebe Junior, Anna Carolina Borges Pereira Costa, Ana Karina da Silva Machado, Juliana Campos Junqueira |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade do Vale do Paraíba -UNIVAP |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
analysis of variance
food.ingredient Time Factors medicine.medical_treatment radiation exposure Photodynamic therapy photosensitizing agent Dental Caries biofilm Microbiology Streptococcus mutans chemistry.chemical_compound food Antimicrobial effect medicine Rose bengal Agar General Materials Science Photosensitizer time Cells Cultured cell culture Analysis of Variance Rose Bengal Photosensitizing Agents biology Strain (chemistry) isolation and purification business.industry bacterial load drug effect methodology Erythrosine biology.organism_classification Molecular biology Bacterial Load lcsh:RK1-715 chemistry Photochemotherapy lcsh:Dentistry Biofilms business |
| Zdroj: | Brazilian Oral Research v.24 n.4 2010 Brazilian Oral Research Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Oral Research, Vol 24, Iss 4, Pp 413-418 (2010) Brazilian Oral Research, Volume: 24, Issue: 4, Pages: 413-418, Published: DEC 2010 |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:48Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:35:54Z : No. of bitstreams: 1 2-s2.0-78650628907.pdf: 173176 bytes, checksum: 22129ff93a68568dee066cb2bad6aa13 (MD5) Made available in DSpace on 2014-05-27T11:24:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-10-01 The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10 6 cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p ≤ 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log 10 in the RB+L+ group and of 5.16 log 10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied. Department of Biosciences and Oral Diagnosis School of Dentistry of São José dos Campos UNESP - Univ. Estadual Paulista, São José dos Campos, SP Instituto de Pesquisa e Desenvolvimento-IP and D Universidade do Vale do Paraíba -UNIVAP, São José dos Campos, SP Department of Biosciences and Oral Diagnosis School of Dentistry of São José dos Campos UNESP - Univ. Estadual Paulista, São José dos Campos, SP |
| Databáze: | OpenAIRE |
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