Quantitative multiplex CARS spectroscopy in congested spectral regions
| Autor: | Müller, M., Rinia, H.A., Bonn, M., Vartiainen, E.M., Lisker, M., Bel, A., Periasamy, A., So, P.T.C. |
|---|---|
| Přispěvatelé: | Molecular Cytology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Multiphoton Microscopy in the Biomedical Sciences VII: 21-23 January 2007, San Jose, California, USA Multiphoton Microscopy in the Biomedical Sciences VII |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.698566 |
| Popis: | A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach, exemplified here with high-quality multiplex CARS data, makes use of spontaneous Raman scattering results. It is shown that the underlying vibrational Raman response can be retrieved from the multiplex CARS spectra, so that the Raman spectrum can be reconstituted, provided an adequate signal-to-noise ratio (SNR) is present in the experimental data and sufficient a priori knowledge of the vibrational resonances involved exists. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of adenosine mono-, di-, and triphosphate (AMP, ADP, and ATP), nicotinamide adenine dinucleotide (NAD+), and small unilamellar vesicles (SUVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Quantitative det... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|