A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer
| Autor: | Fatemeh Molaabasi, Seyed Morteza Naghib, Zahra Rahimzadeh, Kyong Yop Rhee, Ali Sadr, Mandana Afsharpad, Yasser Zare, Esfandyar Askari |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
Materials science Physical and theoretical chemistry QD450-801 Energy Engineering and Power Technology Medicine (miscellaneous) 02 engineering and technology TP1-1185 Conjugated system 010402 general chemistry 01 natural sciences law.invention Biomaterials Circulating tumor cell Breast cancer breast cancer law medicine skin and connective tissue diseases Early breast cancer immunosensor Graphene Process Chemistry and Technology Chemical technology herceptin graphene 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Surfaces Coatings and Films single cell Cancer research 0210 nano-technology Biotechnology |
| Zdroj: | Nanotechnology Reviews, Vol 10, Iss 1, Pp 744-753 (2021) |
| ISSN: | 2191-9097 |
| Popis: | In this paper, we use a simple and cheap approach for the synthesis of herceptin-conjugated graphene biosensor to detect the HER2-positive breast cancer cells. The bifunctional graphene-herceptin nanosheets are prepared from graphite by a simple ultrasonic-mediated technique. The prepared protein-mediated graphene is fully characterized. The results show the exfoliation of graphene layers in herceptin solution. Moreover, herceptin is effectively conjugated into the surface of graphene nanosheets. The synthesized herceptin-conjugated graphene is applied for breast cancer detection. The linear range of this biosensor is 1–80 cells, which is significant. The biosensor shows an excellent selectivity performance for detection of HER2-positive cancer cells. Likewise, the stability and functionality of the biosensor is about 40 days. Based on the results, this device is a promising candidate for rapid and selective detection of cancer cells. |
| Databáze: | OpenAIRE |
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