B-cell antigens within normal and activated human T cells
| Autor: | J Hair, G. P. Sandilands, M Wootton, I. A. R. More, M Perry |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
CD32
Cytoplasm Immunoelectron microscopy Lymphocyte T-Lymphocytes Immunology Cell Culture Techniques Lymphocyte Activation Antigen Antigens CD medicine Immunology and Allergy Humans Microscopy Immunoelectron Pan-T antigens B cell CD antigen B-Lymphocytes biology Original Articles Flow Cytometry Molecular biology Cell biology medicine.anatomical_structure biology.protein |
| Popis: | In this study we compared cell surface staining for human peripheral blood lymphocyte (PBL) CD antigens by flow cytometry, with staining obtained following permeabilization of PBL using the Cytoperm method (Serotec). Six CD antigens (CD20, CD21, CD22, CD32, CD35 and major histocompatibility complex class II antigen) normally found on the surface of B cells, were also found to be expressed within T cells. We also showed, by immunoelectron microscopy, that these inappropriately expressed ('occult') CD antigens are located within cytoplasmic vesicles or within the rough endoplasmic reticulum. Following in vitro activation of T cells a distinct increase in expression of all of these cytoplasmic antigens was observed but staining at the cell surface was, by comparison, weak. We therefore propose that up-regulation of various B-cell CD antigens occurs within the cytoplasm of T cells following activation and that these antigens may be synthesized and released into the fluid-phase as soluble immunoregulatory molecules. |
| Databáze: | OpenAIRE |
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