Effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic Escherichia coli
| Autor: | Z. Fu, R.W. Hoffman, E. N. Miller, M.T. Mullinnix, Zhaohui Tong, Ismael U. Nieves, C.C. Geddes, Lonnie O. Ingram |
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| Rok vydání: | 2011 |
| Předmět: |
Thiosulfate
Environmental Engineering Ethanol Sulfur Compounds Renewable Energy Sustainability and the Environment Sodium chemistry.chemical_element Bioengineering General Medicine Sodium metabisulfite Xylose Furfural Saccharum chemistry.chemical_compound Hydrolysis chemistry Biochemistry Fermentation Escherichia coli Phosphoric Acids Biomass Food science Bagasse Waste Management and Disposal |
| Zdroj: | Bioresource Technology. 102:5145-5152 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2011.02.008 |
| Popis: | The addition of reduced sulfur compounds (thiosulfate, cysteine, sodium hydrosulfite, and sodium metabisulfite) increased growth and fermentation of dilute acid hydrolysate of sugarcane bagasse by ethanologenic Escherichia coli (strains LY180, EMFR9, and MM160). With sodium metabisulfite (0.5 mM), toxicity was sufficiently reduced that slurries of pretreated biomass (10% dry weight including fiber and solubles) could be fermented by E. coli strain MM160 without solid–liquid separation or cleanup of sugars. A 6-h liquefaction step was added to improve mixing. Sodium metabisulfite also caused spectral changes at wavelengths corresponding to furfural and soluble products from lignin. Glucose and cellobiose were rapidly metabolized. Xylose utilization was improved by sodium metabisulfite but remained incomplete after 144 h. The overall ethanol yield for this liquefaction plus simultaneous saccharification and co-fermentation process was 0.20 g ethanol/g bagasse dry weight, 250 L/tonne (61 gal/US ton). |
| Databáze: | OpenAIRE |
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