Isolation of wheat bran-colonizing and metabolizing species from the human fecal microbiota
| Autor: | Filip J. R. Meysman, Silvia Hidalgo Martinez, Kim De Paepe, Mohammad Naser Rezaei, Jeroen Raes, Tom Van de Wiele, Christophe M. Courtin, Davy Van de Walle, Koen Dewettinck, Joran Verspreet |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
BIOFILMS
Firmicutes lcsh:Medicine PROTEIN LARGE-BOWEL Gut flora Wheat bran-utilizing microbiota Microbiology General Biochemistry Genetics and Molecular Biology COLONIZATION Actinobacteria 03 medical and health sciences LACTOBACILLUS INTESTINAL MICROBIOTA Lactobacillus PRAUSNITZII Food science Biology Molecular Biology 030304 developmental biology 0303 health sciences Science & Technology FERMENTATION Insoluble dietary particles biology Bran 030306 microbiology General Neuroscience lcsh:R GUT MICROBIOTA digestive oral and skin physiology Wheat bran-attached microbiota Biology and Life Sciences Bacteroidetes food and beverages General Medicine biology.organism_classification Multidisciplinary Sciences Enrichment BACTERIA Science & Technology - Other Topics Proteobacteria General Agricultural and Biological Sciences Engineering sciences. Technology Bacteria Human gut microbiota |
| Zdroj: | PeerJ PEERJ PeerJ, 7(1) PeerJ, Vol 7, p e6293 (2019) |
| ISSN: | 2167-8359 |
| Popis: | Undigestible, insoluble food particles, such as wheat bran, are important dietary constituents that serve as a fermentation substrate for the human gut microbiota. The first step in wheat bran fermentation involves the poorly studied solubilization of fibers from the complex insoluble wheat bran structure. Attachment of bacteria has been suggested to promote the efficient hydrolysis of insoluble substrates, but the mechanisms and drivers of this microbial attachment and colonization, as well as subsequent fermentation remain to be elucidated. We have previously shown that an individually dependent subset of gut bacteria is able to colonize the wheat bran residue. Here, we isolated these bran-attached microorganisms, which can then be used to gain mechanistic insights in future pure culture experiments. Four healthy fecal donors were screened to account for inter-individual differences in gut microbiota composition. A combination of a direct plating and enrichment method resulted in the isolation of a phylogenetically diverse set of species, belonging to theBacteroidetes,Firmicutes,ProteobacteriaandActinobacteriaphyla. A comparison with 16S rRNA gene sequences that were found enriched on wheat bran particles in previous studies, however, showed that the isolates do not yet cover the entire diversity of wheat-bran colonizing species, comprising among others a broad range ofPrevotella,BacteroidesandClostridiumcluster XIVa species. We, therefore, suggest several modifications to the experiment set-up to further expand the array of isolated species. |
| Databáze: | OpenAIRE |
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