Integrated workflow for urinary prostate specific antigen N-glycosylation analysis using sdAb partitioning and downstream capillary electrophoresis separation
| Autor: | András Guttman, Hajnalka Jankovics, Eszter Gacsi, Tibor Szarvas, Gabor Jarvas, Balazs Reider, Ferenc Vonderviszt |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Glycan Glycosylation Medizin Biochemistry Analytical Chemistry Workflow Prostate cancer Capillary electrophoresis Antigen N-linked glycosylation medicine Environmental Chemistry Humans Spectroscopy biology Chemistry Cancer Electrophoresis Capillary Prostatic Neoplasms Prostate-Specific Antigen medicine.disease Prostate-specific antigen Single-domain antibody Cancer research biology.protein |
| Popis: | Prostate cancer represents the second highest malignancy rate in men in all cancer diagnoses worldwide. The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. In this paper we report on the development and implementation of a high-throughput capillary electrophoresis based glycan analysis workflow for urinary PSA analysis. The technology utilizes selective, high yield single domain antibody based PSA capture, followed by preconcentration and capillary electrophoresis coupled with laser-induced fluorescence detection, resulting in high resolution N-glycan profiles. Urinary PSA glycan profiles were compared to a commercially available PSA standard revealing differences in their α2,3- and α2,6-sialylated isomers, proving the excellent selectivity of the suggested workflow. This is important as sialylation classification plays an important role in the differentiation between indolent, significant and aggressive forms of prostate cancer. |
| Databáze: | OpenAIRE |
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